Identification and Characterization of the Two-Component System HK8700–RR8701 of Kocuria rhizophila DC2201
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Two-component systems (TCSs) are highly conserved in prokaryotes, endowing cells with multiple physiological functions to respond to changes in the ambient environment. The signaling pathway of a typical TCS consists of a sensory histidine kinase and a response regulator. The TCSs of Kocuria rhizophila, which is usually used as a target strain for various antibiotics and other adverse factors, have captured our interest due to their potential roles in bacterial adaptation for survival. Herein, the distribution and putative biological functions of the TCSs of K. rhizophila DC2201 were analyzed by using bioinformatics, and a preliminary TCS regulatory network was constructed. A representative and important TCS (i.e., HK8700–RR8701 system), which is homologous to the LiaS–LiaR system previously discovered in Bacillus subtilis, was identified and characterized through yeast two-hybrid screening and phosphorylation assays. Detailed information of TCSs is expected to offer novel insights into the adaptation mechanism of K. rhizophila and thus boost its application.
KeywordsKocuria rhizophila Two-component system HK8700–RR8701 Yeast two-hybrid system Signal transduction
We deeply appreciate Liangguo Xu for assistance with strains and plasmids. This work was supported by the National Natural Science Foundation of China (Grant No. 31360018), Key R&D Program Projects of Jiangxi Province, China (Grant No. 20161BBF60076), and the Innovation Program for Graduates of Jiangxi Normal University (Grant No. YJS2018081).
ZL designed the study. BC and TZ performed the experiments. YH and HN contributed to the results analysis and discussion. BC wrote the manuscript, and ZL and LZ revised the manuscript. All the authors approved the paper.
Compliance with Ethical Standards
Conflict of interest
The authors have declared that they have no competing interests.
This article does not contain any studies with human participants or animals performed by any of the authors.
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