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The Protein Journal

, Volume 38, Issue 5, pp 586–597 | Cite as

Characterization of Protein Profiling and mRNA Expression of LLC Exosomes

  • Wen Zhang
  • Pei He
  • Shibei Wang
  • Amila Adili
  • Zixuan Chen
  • Chen-Yu Zhang
  • Xiaohong JiangEmail author
  • Jing LiEmail author
  • Yujing ZhangEmail author
Article
  • 227 Downloads

Abstract

Circulating exosomes are promising biomarker source in various diseases. Exosomal constituents can stably exist in the circulating plasma and serum thus making them ideal biomarkers for a number of clinical applications. Exosomes can also mediate the occurrence of many types of diseases, including distal cancerous metastasis and tumour enlargement, through encapsulated proteins or RNAs, which regulate interactions among tissues. While performing these actions, exosomes show tissue specificity. However, the mechanism for such selection is not clear. For non-small cell lung cancer (NSCLC), molecular diagnostic markers and mechanisms of exosome-mediated tumour metastasis are not well understood. Therefore, in this study, we characterized LLC exosomal proteins and mRNAs by analysing their molecular profiles, laying a foundation for exploring diagnostic markers of lung cancer. Furthermore, the interactions between exosomal membrane proteins and their target proteins were analysed and revealed a possible tissue propensity of LLC cell-derived exosomes. These findings provide a theoretical basis for studying exosome-mediated tissue targeting and distal lung cancer metastasis.

Keywords

LLC Exosome Proteomics Transcriptomics Target tissue analysis 

Notes

Acknowledgements

We thank for Pei He and Shibei Wang collecting exosomes of LLC cells and running assays for following bioinformatics investigations in this study. We thanks Yujing Zhang designing and supervising this work.

Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 31771666, 31741066, 31200969) and the Industrialization of Mongolian Medicine of “Prairie Talents” engineering (NEI ZU TONG ZI No. 2015-56).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10930_2019_9849_MOESM1_ESM.xlsx (63 kb)
Supplementary material 1 (XLSX 62 kb). A total of 1035 LLC cell-derived exosomal proteins were identified by LC-MS/MS.
10930_2019_9849_MOESM2_ESM.csv (16 kb)
Supplementary material 2 (CSV 15 kb). Detailed relationships of LLC exosomal protein PPI network.
10930_2019_9849_MOESM3_ESM.xlsx (132 kb)
Supplementary material 3 (XLSX 131 kb). A total of 2531 LLC cell-derived exosomal mRNAs were identified by microarray.
10930_2019_9849_MOESM4_ESM.xlsx (16 kb)
Supplementary material 4 (XLSX 16 kb). Detailed relationships of LLC exosomal mRNA PPI network.
10930_2019_9849_MOESM5_ESM.xlsx (10 kb)
Supplementary material 5 (XLSX 9 kb). Proteins involved in the signaling pathway.
10930_2019_9849_MOESM6_ESM.xlsx (11 kb)
Supplementary material 6 (XLSX 10 kb). Target tissue analysis of proteins.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Pharmaceutical Biotechnology, Collaborative Innovation Center of Chemistry for Life Sciences, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life SciencesNanjing UniversityNanjingChina
  2. 2.Innovative Mongolian Medical Engineering Research CentreInternational Mongolian Hospital of Inner MongoliaHohhotChina

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