The Protein Journal

, Volume 37, Issue 4, pp 324–332 | Cite as

Identification and Functional Analysis of Novel Bradykinin-Related Peptides (BRPs) from Skin Secretions of Five Asian Frogs

  • Baowen Zhang
  • Xiaoli Zhang
  • Ying Yang
  • Yuhong HuEmail author
  • Hui WangEmail author


In recent decades, various types of bioactive substances have been identified from amphibian skin and its secretions. Bradykinin-related peptides (BRPs) are among these compounds that make up the host defence system of amphibians. In the present study, we identified six novel BRPs, amolopkinin-GN1, amolopkinin-RK1, amolopkinin-TR1, amolopkinin-LF1, ranakinin-MS1, and ranakinin-MS2, from five East Asian amphibians, Amolops granulosus, Amolops ricketti, Amolops torrentis, Amolops lifanensis, and Hylarana maosonensis. This is the first report on BRPs in the skin of these species. Physiological assays reveal that these peptides have a contractive effect on the smooth muscle of rat ileum.


Amphibian Skin Bradykinin-related peptides Host defence 



The study was supported by the Natural Science Foundation of Educational Committee of Hebei Province of China (Nos. QN2016129), Natural Science Foundation of Hebei Province of China (Nos. C2016205244), and National Natural Science Foundation of China (Nos. 31200309).

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflicts of interest.

Ethical Approval

This article does not contain any studies with human participants performed by any of the authors.


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Authors and Affiliations

  1. 1.Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life SciencesHebei Normal UniversityShijiazhuangChina
  2. 2.Instrumental Analysis CenterHebei Normal UniversityShijiazhuangChina

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