The Protein Journal

, Volume 37, Issue 3, pp 237–247 | Cite as

In Silico Analysis of Natural Resistance-Associated Macrophage Protein (NRAMP) Family of Transporters in Rice

  • Anitha Mani
  • Kavitha Sankaranarayanan


In Oryza sativa (rice) there are seven members in the NRAMP (natural resistance- associated macrophage protein) family of transporter proteins. They have been identified as OsNRAMP1, OsNRAMP2, OsNRAMP3, OsNRAMP4, OsNRAMP5, OsNRAMP6 and OsNRAMP7. Several metal ions like Zn2+, Mn2+, Fe2+, Cd2+ etc. have been studied to be transported via NRAMP transporter proteins in rice plant. In spite of this, very little information is available regarding these transporters. Hence it is important to computationally predict and characterize the OsNRAMP family of transporters for studying and understanding their molecular insights in future studies. For this purpose, various in silico methods and tools were used for the characterization of OsNRAMP family of transporter proteins. Physico-chemical properties of the protein sequences were calculated, putative transmembrane domains (TMDs) and conserved motif signatures were determined and their interaction partners were predicted. 3D models of all the members of OsNRAMP transporters were generated using online structure prediction tool followed by their analysis. In silico microarray analysis was done to understand the expression pattern of these transporters in rice plant. Currently, only limited knowledge is available about the structural and functional aspects of these transporters, hence this study would provide more theoretical information about them.


Oryza sativa Physico chemical features Interaction partners 3D model prediction Gene expression analysis 



AM would like to thank T. Manonanthini, Bioinformatics lab, AU-KBC Research Centre for her help. AM would like to thank Anna University for providing Anna Centenary Research Fellowship during the research work.

Compliance with Ethical Standards

Conflict of interest

Both the authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10930_2018_9773_MOESM1_ESM.pdf (2 mb)
Supplementary material 1 (PDF 2047 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Ion Channel Biology Laboratory, AU-KBC Research CentreMadras Institute of Technology, Anna UniversityChennaiIndia

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