Trans-sialidase Protein as a Potential Serological Marker for African Trypanosomiasis
- 30 Downloads
Trypanosoma brucei is the etiological agent of African trypanosomiasis responsible for human and animal infections. T. brucei is transmitted by infected tsetse flies. There is no vaccine for the disease and drugs available for treatment are inefficient and high toxicity. In this context, it is a priority to find antigenic targets suitable for the development of new diagnostic tools, drugs and vaccines. In this work, we report that mice infected with T. b. brucei produce antibodies against trans-sialidase recombinant protein (TS). In addition, we also demonstrate that bloodstream T. b. brucei express messenger RNA related to the TS gene. Collectively, our data strongly suggest that bloodstream forms of T. b. brucei also express the TS gene, that to date was described only in the procyclic forms of the T. b. brucei. In conclusion, these results highlight the importance of TS protein as a possible antigen target during infection caused by T. b. brucei.
KeywordsTrans-sialidase Trypanosoma brucei African trypanosomiasis Antigenic targets Serological markers
Thanks to Global Health and Tropical Medicine (GHTM-UID/multi/04413/2013) by support and the Programa Ciências Sem Fronteiras, Capes—Brazil (Grant No. 019/2013). We are also grateful to Paulo Fanado for editing this manuscript.
Conceived and designed the experiments: AFT and MSS; Performed the experiments: AFT, JGP, and SPA; Analysed the data: AFT, JGP, SPA, and MSS; Wrote the paper: AFT and MSS. All authors read and approved the final version of the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
Murine model was performed in accordance with existing legislation in Portugal and in accordance of the Ethical Committee at Institute of Hygiene and Tropical Medicine, Lisbon—Portugal.
- 3.Ponte-Sucre A (2016) An overview of Trypanosoma brucei infections: an intense host-parasite interaction. Front Microbiol 7(2126):1–12Google Scholar
- 7.Pontes de Carvalho, LC, Tomlinson S, Vandekerckhove F, Bienen EJ, Clarkson AB, Jiang MS, Hart GW, Nussenzweig V (1993) Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. J Exp Med 177(2):465–474CrossRefGoogle Scholar
- 12.Perreira-Chioccola VL, Fragata-Filho AA, Levy AM, Rodrigues MM, Schenkman S (2003) Enzyme-linked immunoassay using recombinant trans-sialidase of Trypanosoma cruzi can be employed for monitoring of patients with Chagas’ disease after drug treatment. Clin Diagn Lab Immunol 10(5):826–830Google Scholar
- 21.World Health Organization (2007) Report of a WHO informal consultation on sustainable control of human African trypanosomiasis. Geneva, SwitzerlandGoogle Scholar