The Protein Journal

, Volume 32, Issue 6, pp 449–455 | Cite as

The RNA Chaperone and Protein Chaperone Activity of Arabidopsis Glycine-Rich RNA-Binding Protein 4 and 7 is Determined by the Propensity for the Formation of High Molecular Weight Complexes

  • Ji Hoon Han
  • Young Jun Jung
  • Hyun-Ju Lee
  • Hyun Suk Jung
  • Kyun Oh Lee
  • Hunseung Kang
Article

Abstract

RNA chaperones and protein chaperones are cellular proteins that can aid the correct folding of target RNAs and proteins, respectively. Although many proteins possessing RNA chaperone or protein chaperone activity have been demonstrated in diverse organisms, report evaluating the RNA chaperone and protein chaperone activity of a given protein is severely limited. Here, two glycine-rich RNA-binding proteins in Arabidopsisthaliana (AtGRPs), AtGRP7 exhibiting RNA chaperone activity and AtGRP4 exhibiting no RNA chaperone activity, were investigated for their protein chaperone activity. The heat-induced thermal aggregation of a substrate protein was significantly decreased with the addition of AtGRP4 depending on protein concentration, whereas the thermal aggregation of a substrate protein was further increased with the addition of AtGRP7, demonstrating that AtGRP4 but not AtGRP7 possesses protein chaperone activity. Size exclusion chromatography and electron microscopy analyses revealed that the formation of high molecular weight (HMW) complexes is closely related to the protein chaperone activity of AtGRP4. Importantly, the additional 25 amino acids at the N-terminus of AtGRP4 are crucial for HMW complex formation and protein chaperone activity. Taken together, these results show that the formation of HMW complexes is important for determining the RNA chaperone and protein chaperone activity of AtGRP4 and AtGRP7.

Keywords

Arabidopsis thaliana Glycine-rich RNA-binding protein Protein chaperone Protein folding RNA chaperone RNA folding 

Abbreviations

AtGRP4

Arabidopsis thaliana GRP4

AtGRP7

Arabidopsis thaliana GRP7

GRP

Glycine-rich RNA-binding protein

HMW

High molecular weight

MDH

Malate dehydrogenase

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ji Hoon Han
    • 1
  • Young Jun Jung
    • 2
  • Hyun-Ju Lee
    • 3
  • Hyun Suk Jung
    • 3
  • Kyun Oh Lee
    • 2
  • Hunseung Kang
    • 1
  1. 1.Department of Plant Biotechnology, College of Agriculture and Life SciencesChonnam National UniversityKwangjuKorea
  2. 2.Division of Applied Life Science (BK21 Program) and PMBBRCGyeongsang National UniversityJinjuKorea
  3. 3.Division of Electron Microscopic ResearchKorea Basic Science InstituteTaejonKorea

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