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The Protein Journal

, Volume 32, Issue 3, pp 208–215 | Cite as

A Calcium Ion-Dependent Dimeric Bean Lectin with Antiproliferative Activity Toward Human Breast Cancer MCF-7 Cells

  • Randy Chi Fai Cheung
  • Ho Him Leung
  • Wen Liang Pan
  • Tzi Bun Ng
Article

Abstract

In this study, a 60.8-kDa dimeric lectin was isolated from the Phaseolus vulgaris cv. jade bean and characterized. The lectin was bound on Blue Sepharose 6 and Q Sepharose and was finally purified by size exclusion chromatography on Superdex 200. Its hemagglutinating activity toward rabbit erythrocytes was dependent on divalent cations, especially calcium ions. Various carbohydrates tested were devoid of any effect on the hemagglutinating activity. The lectin was stable at pH between 4.5 and 9.4 and temperatures between 30 and 70 °C. It did not exert antifungal activity toward Valsa mali, Setosphaeria turcica, Mycosphaerella arachidicola, Fusarium oxysporum and Bipolaris maydis. The IC50 of the antiproliferative activity of the lectin toward MCF-7 human breast cancer cells was 174 μM. It did not inhibit proliferation of WRL-68 human normal embryonic hepatocytes. The lectin was dependent on calcium ions for hemagglutinating activity and possessed a blocked N-terminus. These two characteristics make the lectin unique among Phaseolus lectins.

Keywords

Phaseolus vulgaris Lectin Antitumor Purification Breast cancer cells 

Abbreviations

FPLC-SEC

Fast protein liquid chromatography-size exclusion chromatography

HA

Hemagglutinating activity

IC50

The half maximal inhibitory concentration

ML

Mistletoe lectin

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Randy Chi Fai Cheung
    • 1
  • Ho Him Leung
    • 2
  • Wen Liang Pan
    • 1
  • Tzi Bun Ng
    • 1
  1. 1.School of Biomedical Sciences, Faculty of MedicineThe Chinese University of Hong KongHong KongChina
  2. 2.Department of MedicineImperial College LondonLondonUK

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