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The Protein Journal

, Volume 31, Issue 6, pp 477–486 | Cite as

The Histone H1 Variant Accumulates in Response to Water Stress in the Drought Tolerant Genotype of Gossypium herbaceum L.

  • Ila Trivedi
  • Alok Ranjan
  • Y. K. Sharma
  • Samir Sawant
Article

Abstract

We have optimized and improved the protocol for extraction of histone proteins from Gossypium herbaceum. Histone proteins were isolated by acid extraction method and fractionation of histone proteins were performed using RP-HPLC (reverse-phase high performance liquid chromatography). Analysis of histones from drought tolerant (Vagad) and drought sensitive genotype (RAHS-14) indicated that the tolerant genotype Vagad encodes a 29 kDa protein. Protein sequencing on MALDI TOF/TOF revealed that the 29 kDA protein shared sequence similarity with another drought-inducible linker histone-H1.S reported in tomato. This H1.S like linker histone was not found in RAHS-14 in our study. We further examined the expression of H1 variant at the transcript and protein levels and found that it was induced specifically in the tolerant genotype Vagad.

Keywords

Histone variants Gossypium herbaceum Genotype Chromatin 

Abbreviations

RP-HPLC

Reverse-phase high performance liquid chromatography

w/v

Weight/volume

Tris

Tris (hydroxymethyl) aminomethane

EDTA

Ethylenediaminetetraacetic acid

SDS-PAGE

Sodium dodecyl sulphate–polyacrylamide gel electrophoresis

PVDF

Polyvinylidene difluoride

MALDI-TOF

Matrix-assisted laser desorption/ionization- time-of-flight

RT-PCR

Real-time PCR

Notes

Acknowledgments

This work was supported by a research grant received from the Council of Scientific and Industrial Research (CSIR), Government of India. IT and AR acknowledge CSIR for research fellowship.

Supplementary material

10930_2012_9425_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 11 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ila Trivedi
    • 1
  • Alok Ranjan
    • 1
  • Y. K. Sharma
    • 2
  • Samir Sawant
    • 1
  1. 1.Plant Molecular Biology and Genetic Engineering DivisionCSIR, National Botanical Research InstituteLucknowIndia
  2. 2.Department of BotanyFaculty of Science, University of LucknowLucknowIndia

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