The Protein Journal

, Volume 31, Issue 6, pp 439–446 | Cite as

Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action

Article

Abstract

Garlic (Allium sativum) lectins are promising candidate molecules for the protection against chewing (lepidopteran) as well as sap sucking (homopteran) insect pests. Molecular mechanism of toxicity and interaction of lectins with midgut receptor proteins has been described in many reports. Lectins show its effect right from sensory receptors of mouth parts by disrupting the membrane integrity and food detection ability. Subsequently, enter into the gut lumen and interact with midgut glycosylated proteins like alkaline phosphatase (ALP), aminopeptidase-N (APN), cadherin-like proteins, polycalins, sucrase, symbionin and others. These proteins play critical role in life cycle of insect directly or indirectly. Lectins interfere with the activity of these proteins and causes physiological disorders leading to the death of insects. Lectins further transported across the insect gut, accumulated in various body parts (like haemolymph and ovary) and interact with intracellular proteins like symbionin and cytochrome p450. Binding with cytochrome p450 (which involve in ecdysone synthesis) might interfere in the development of insects, which results in growth retardation and pre-mature death.

Keywords

Garlic lectins Glycosylated Homopteran Insect Lepidopteran 

Abbreviations

ALP

Alkaline Phosphatase

APN

Aminopeptidase-N

ASA

Allium sativum agglutinin

ASAL

Allium sativum leaf agglutinin

GNA

Galanthus nivalis agglutinin

BBMV

Brush border membrane vesicles

Notes

Acknowledgment

Authors are grateful to the Council of Scientific and Industrial Research, Government of India for funding the research. SKU is thankful to CSIR for senior research fellowship and G B Technical University, Lucknow, UP, India for Ph.D. registration.

References

  1. 1.
    Andersson JO (2000) Curr Biol 10:R866–R868CrossRefGoogle Scholar
  2. 2.
    Angelucci C, Barrett-Wilt GA, Hunt DF, Akhurst RJ, East PD, Gordon KH, Campbell PM (2008) Insect Biochem Mol Biol 38:685–696CrossRefGoogle Scholar
  3. 3.
    Angst BD, Marcozzi C, Magee AI (2001) J Cell Sci 114:629–641Google Scholar
  4. 4.
    Arenas I, Bravo A, Soberon M, Gomez I (2010) J Biol Chem 285:12497–12503CrossRefGoogle Scholar
  5. 5.
    Arora A, Sharma HC, Dreissche EV, Sharma KK (2005) SAT eJournal/ejournal.icrisat.org. 1:1–3Google Scholar
  6. 6.
    Ashford DA, Smith WA, Douglas AE (2000) J Insect Physiol 46:335–341CrossRefGoogle Scholar
  7. 7.
    Bachhawat K, Thomas CJ, Amutha B, Krishnasastryi MV, Khan MI, Surolia A (2001) J Biol Chem 276:5541–5546CrossRefGoogle Scholar
  8. 8.
    Bandyopadhyay S, Roy A, Das S (2001) Plant Sci 161:1025–1033CrossRefGoogle Scholar
  9. 9.
    Banerjee S, Hess D, Majumder P, Roy D, Das S (2004) J Biol Chem 279:23782–23789CrossRefGoogle Scholar
  10. 10.
    Bharathi Y, VijayaKumar S, Pasalu IC, Balachandran SM, Reddy VD, Rao KV (2011) J Biotechnol 152:63–71CrossRefGoogle Scholar
  11. 11.
    Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, Van Die I, Burton DR, Wilson IA, Cummings R, Bovin N, Wong CH, Paulson JC (2004) Proc Natl Acad Sci USA 101:17033–17038CrossRefGoogle Scholar
  12. 12.
    Burton SL, Ellar DJ, Li J, Derbyshire DJ (1999) J Mol Biol 287:1011–1022CrossRefGoogle Scholar
  13. 13.
    Carlini CR, Grossi-de-Sab (2002) Toxicon 40:1515–1539CrossRefGoogle Scholar
  14. 14.
    Chakraborti D, Sarkar A, Mondal HA, Das S (2009) Transgenic Res 18:529–544CrossRefGoogle Scholar
  15. 15.
    Chandra NR, Ramachandraiah G, Bachhawat K, Dam TK, Surolia A, Vijayan M (1999) J Mol Biol 285:1157–1168CrossRefGoogle Scholar
  16. 16.
    Chen SJ, Chen NT, Wang SH, Hsu JC, Ding WH, Kuo-Huang LL, Huang RN (2009) Pest Manag Sci 65:923–930CrossRefGoogle Scholar
  17. 17.
    Cristofoletti PT, Ribeiro AF, Deraison C, Rahbe′ Y, Terra WR (2003) J Insect Physiol 49:11–24CrossRefGoogle Scholar
  18. 18.
    Dam TK, Bachhawat K, Rani PG, Surolia A (1998) J Biol Chem 273:5528–5535CrossRefGoogle Scholar
  19. 19.
    Denolf P, Hendrickx K, Van Damme J, Jansens S, Peferoen M, Degheele D, Van Rie J (1997) Eur J Biochem 248:748–761CrossRefGoogle Scholar
  20. 20.
    Downing N (1978) J Exp Biol 77:247–250Google Scholar
  21. 21.
    Dunne J, Hanby AM, Poulsom R, Jones TA, Sheer D, Chin WG, Da SM, Zhao Q, Beverley PC, Owen MJ (1995) Genomics 30:207–223CrossRefGoogle Scholar
  22. 22.
    Dutt U (2007) Environment News Service, 21 August, (countercurrents.org)Google Scholar
  23. 23.
    Dutta IP, Majumdar P, Saha P, Ray K, Das S (2005) Plant Sci 169:996–1007CrossRefGoogle Scholar
  24. 24.
    Dutta I, Saha P, Majumder P, Sarkar A, Chakraborti D, Banerjee S, Das S (2005) Plant Biotech J 3:601–611CrossRefGoogle Scholar
  25. 25.
    Filichkin SA, Brumfield S, Filichkin TP, Young MJ (1997) J Virol 71:569–577Google Scholar
  26. 26.
    Fisher DB (2000) In: Gruissem W, Jones RL, Buchanan BB (eds) Long distance transport. American Society of Plant Physiologists, Rockville, pp 730–784Google Scholar
  27. 27.
    Fitches E, Gatehouse AMR, Gatehouse JA (1997) J Insect Physiol 43:727–739CrossRefGoogle Scholar
  28. 28.
    Fitches E, Ilett C, Gatehouse AMR, Gatehouse LN, Greene R, Edwards JP, Gatehouse JA (2001) J Insect Physiol 47:1389–1398CrossRefGoogle Scholar
  29. 29.
    Fitches E, Philip J, Hinchliffe G, Vercruysse L, Chougule N, Gatehouse JA (2008) Insect Sci 15:483–495CrossRefGoogle Scholar
  30. 30.
    Fitches E, Wiles D, Douglas AE, Hinchliffe G, Audsley N, Gatehouse JA (2008) Insect Biochem Mol Biol 38:905–915CrossRefGoogle Scholar
  31. 31.
    Gatehouse AMR, Dewey FM, Dove J, Fenton KA, Pusztai A (1984) J Sci Food Agric 35:373–380CrossRefGoogle Scholar
  32. 32.
    Gatehouse AMR, Powell KS, Van Damme EJM, Peumans W, Gatehouse JA (1995) In: Pusztai AJ, Bardocz S (eds) Lectins: biomedical perspectives. Taylor & Francis, London, pp 35–57Google Scholar
  33. 33.
    Gilbert LI, Warren JT (2005) Vitam Horm 73:31–57CrossRefGoogle Scholar
  34. 34.
    Goldberg M, Peshkovsky C, Shifteh A, Al-Awqati Q (2000) J Biol Chem 275:24622–24629CrossRefGoogle Scholar
  35. 35.
    Gómez I, Sánchez J, Miranda R, Bravo A, Sánchez M (2002) FEBS Lett 513:242–246CrossRefGoogle Scholar
  36. 36.
    Gumbiner BM (1996) Cell 84:345–357CrossRefGoogle Scholar
  37. 37.
    Habibi J, Backus EA, Czapla TC (1998) Cell Tissue Res 294:561–571CrossRefGoogle Scholar
  38. 38.
    Habibi J, Backus EA, Huesing JE (2000) J Insect Physiol 46:611–619CrossRefGoogle Scholar
  39. 39.
    Harper MS, Hopkins TL, Czapla TH (1998) Tissue Cell 30:166–176CrossRefGoogle Scholar
  40. 40.
    Hilder VA, Powell KS, Gatehouse AMR, Gatehouse JA, Gatehouse LN, Shi Y, Hamilton WDO, Merryweather A, Newell CA, Timans JC (1995) Transgenic Res 4:18–25CrossRefGoogle Scholar
  41. 41.
    Hooper NM (1994) FEBS Lett 354:1–6CrossRefGoogle Scholar
  42. 42.
    Hossain DM, Shitomi Y, Moriyama K, Higuchi M, Hayakawa T, Mitsui T, Sato R, Hori H (2004) Appl Environ Microbiol 70:4604–4612CrossRefGoogle Scholar
  43. 43.
    Hua G, Zhang R, Abdullah MA, Adang MJ (2008) Biochemistry 47:5101–5110CrossRefGoogle Scholar
  44. 44.
    Ingle SS, Trivedi N, Prasad R, Kuruvilla J, Rao KK, Chhatpar HS (2001) Curr Microbiol 43:255–259CrossRefGoogle Scholar
  45. 45.
    Jurat-Fuentes JL, Adang MJ (2004) Euro J Biochem 271:3127–3135CrossRefGoogle Scholar
  46. 46.
    Jurat-Fuentes JL, Adang MJ (2007) J Invert Pathol 95:187–191CrossRefGoogle Scholar
  47. 47.
    Karley AJ, Ashford DA, Minto LB, Pritchard J, Douglas AE (2005) J Insect Physiol 51:1313–1319CrossRefGoogle Scholar
  48. 48.
    Kaur M, Singh K, Rup PJ, Kamboj SS, Saxena AK, Sharma M, Bhagat M, Sood SK, Singh J (2006) J Biochem Mol Biol 39:432–440CrossRefGoogle Scholar
  49. 49.
    Knight PJ, Crickmore N, Ellar DJ (1994) Mol Microbiol 11:429–436CrossRefGoogle Scholar
  50. 50.
    Knight PJ, Knowles BH, Ellar DJ (1995) J Biol Chem 270:17765–17770CrossRefGoogle Scholar
  51. 51.
    Krishnamoorthy M, Jurat-Fuentes JL, McNall RJ, Andacht T, Adang MJ (2007) Insect Biochem Mol Biol 37:189–201CrossRefGoogle Scholar
  52. 52.
    Laustsen PG, Vang S, Kristensen T (2001) Eur J Biochem 268:98–104CrossRefGoogle Scholar
  53. 53.
    Lu YJ, Adang MJ (1996) Insect Biochem Mol Biol 26:33–40CrossRefGoogle Scholar
  54. 54.
    Majumder P, Banerjee S, Das S (2004) Glycoconjugate J 20:525–530CrossRefGoogle Scholar
  55. 55.
    Majumder P, Mondal HA, Das S (2005) J Agric Food Chem 53:6725–6729CrossRefGoogle Scholar
  56. 56.
    Malik K, Mahmood T, Riazuddin S (2001) J Biol Sci 1:782–784CrossRefGoogle Scholar
  57. 57.
    Mauchamp B, Royer C, Garel A, Jalabert A, Rocha MD, Grenier AM, Labas V, Vinh J, Mita K, Kadono K, Chavancy G (2006) Insect Biochem Mol Biol 36:623–633CrossRefGoogle Scholar
  58. 58.
    McNall RJ, Adang MJ (2003) Insect Biochem Mol Biol 33:999–1010CrossRefGoogle Scholar
  59. 59.
    Mondal HA, Chakraborti D, Majumder P, Roy P, Roy A, Bhattacharya SG, Das S (2011) PLoS ONE 6:e27716CrossRefGoogle Scholar
  60. 60.
    Nakayama M, Nakajima D, Nagase T, Nomura N, Seki N, Ohara O (1998) Genomics 51:27–34CrossRefGoogle Scholar
  61. 61.
    Nollet F, Kools P, Roy VF (2000) J Mol Biol 299:551–572CrossRefGoogle Scholar
  62. 62.
    Ohizumi Y, Gaidamashvili M, Ohwada S, Matsuda K, Kominami J, Nakamura-Tsuruta S, Hirabayashi J, Naganuma T, Ogawa T, Muramoto K (2009) J Agric Food Chem 57:2896–2902CrossRefGoogle Scholar
  63. 63.
    Peumans WJ, Van Damme EJM (1995) Plant Physiol 109:347–352CrossRefGoogle Scholar
  64. 64.
    Pigott CR, Ellar DJ (2007) Microbiol Mol Biol Rev 71:255–281CrossRefGoogle Scholar
  65. 65.
    Powell KS (2001) Entomol Exp Appl 99:71–78CrossRefGoogle Scholar
  66. 66.
    Powell KS, Gatehouse AMR, Hilder VA, van Damme EJM, Peumans WJ, Boonjawat J, Horsham K, Gatehouse JA (1995) Entomol Exp Appl 75:61–65CrossRefGoogle Scholar
  67. 67.
    Powell KS, Spence J, Bharathi M, Gatehouse JA, Gatehouse AMR (1998) J Insect Physiol 44:529–539CrossRefGoogle Scholar
  68. 68.
    Ramachandraiah G, Chandra NR (2000) Proteins Struc Func Genet 39:358–364CrossRefGoogle Scholar
  69. 69.
    Ramachandraiah G, Chandra NR, Surolia A, Vijayan M (2002) Acta Cryst D58:414–420Google Scholar
  70. 70.
    Ramachandraiah G, Chandra NR, Surolia A, Vijayan M (2003) Glycobiology 13:765–775CrossRefGoogle Scholar
  71. 71.
    Rao KV, Rathore KS, Hodges TK, Fu X, Stoger E, Sudhakar D, Williams S, Christou P, Bharathi M, Bown DP, Powell KS, Spence J, Gatehouse AM, Gatehouse JA (1998) Plant J 15:469–477CrossRefGoogle Scholar
  72. 72.
    Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Insect Biochem Mol Biol 36:188–199CrossRefGoogle Scholar
  73. 73.
    Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Mol Cell Endocrinol 247:166–174CrossRefGoogle Scholar
  74. 74.
    Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Biochem Soc Trans 34:1256–1260CrossRefGoogle Scholar
  75. 75.
    Rewitz KF, Connor MB, Gilbert LI (2007) Insect Biochem Mol Biol 37:741–753CrossRefGoogle Scholar
  76. 76.
    Rhodes JD, Croghan PC, Dixon AFG (1997) Physiol Entomol 22:373–379CrossRefGoogle Scholar
  77. 77.
    Sadeghi A, Smagghe G, Broeders S, Hernalsteens JP, DeGreve H, Peumans WJ, VanDamme EJ (2008) Transgenic Res 17:9–18CrossRefGoogle Scholar
  78. 78.
    Saha P, Dasgupta I, Das S (2006) Plant Mol Biol 62:735–752CrossRefGoogle Scholar
  79. 79.
    Sarkar A, Hess D, Mondal HA, Banerjee S, Sharma HC, Das S (2009) J Prot Res 8:1838–1848CrossRefGoogle Scholar
  80. 80.
    Sauvion N, Charles H, Febvay G, Rahbe Y (2004) Entomol Exp Appl 110:31–44CrossRefGoogle Scholar
  81. 81.
    Smeets K, Van Damme EJM, Verhaert P, Barre A, Rouge P, Van Leuven F, Peumans WJ (1997) Plant Mol Biol 33:223–234CrossRefGoogle Scholar
  82. 82.
    Takesue S, Yokota K, Miyajima S, Taguchi R, Ikezawa H, Takesue Y (1992) Comp Biochem Physiol B 102:7–11CrossRefGoogle Scholar
  83. 83.
    Upadhyay SK, Chandrashekar K, Thakur N, Verma PC, Borgio JF, Singh PK, Tuli R (2011) J Biosci 36:153–161CrossRefGoogle Scholar
  84. 84.
    Upadhyay SK, Mishra M, Singh H, Ranjan A, Chandrashekar K, Verma PC, Singh PK, Tuli R (2010) Proteomics 10:4431–4440CrossRefGoogle Scholar
  85. 85.
    Upadhyay SK, Saurabh S, Rai P, Singh R, Chandrashekar K, Verma PC, Singh PK, Tuli R (2010) J Biotechnol 146:1–8CrossRefGoogle Scholar
  86. 86.
    Upadhyay SK, Saurabh S, Singh R, Rai P, Dubey NK, Chandrashekar K, Negi KS, Singh PK, Tuli R (2011c) Protein J. doi: 10.1007/s10930-011-9342-0
  87. 87.
    Upadhyay SK, Singh P K (2011b) Biotechnol Lett. doi:  10.1007/s10529-011-0665-x
  88. 88.
    Upadhyay SK, Singh S, Chandrashekar K, Tuli R, Singh P K (2012) Appl Microbiol Biotechnol. doi:  10.1007/s00253-011-3547-1
  89. 89.
    Usui T, Shima Y, Shimada Y, Hirano S, Burgess RW, Schwarz TL, Takeichi M, Uemura T (1999) Cell 98:585–595CrossRefGoogle Scholar
  90. 90.
    Vadlamudi RK, Ji TH, Bulla LA Jr (1993) J Biol Chem 268:12334–12340Google Scholar
  91. 91.
    Vadlamudi RK, Weber E, Ji I, Ji TH, Bulla LA Jr (1995) J Biol Chem 270:5490–5494CrossRefGoogle Scholar
  92. 92.
    Van Damme EJM, Barre A, Rougé P, Peumans WJ (2004) Trends Plant Sci 9:484–489CrossRefGoogle Scholar
  93. 93.
    Van Damme EJM, Lannoo N, Peumans WJ (2008) Adv Bot Res 48:107–209CrossRefGoogle Scholar
  94. 94.
    Van Damme EJM, Peumans WJ, Barre A, Rougé P (1998) CRC Crit Rev Plant Sci 17:575–692CrossRefGoogle Scholar
  95. 95.
    Van Damme EJM, Smeets K, Torrekens S, Van Leuven F, Goldstein IJ, Peumans WJ (1992) Eur J Biochem 206:413–420CrossRefGoogle Scholar
  96. 96.
    Van Driessche E, Fischer J, Beeckmans S, Bog-Hanse TC (1996) Lectins, biology, biochemistry, clinical biochemistry, Textop, Denmark, vol 11Google Scholar
  97. 97.
    Vasconcelos IM, Oliveira JTA (2004) Toxicon 44:385–403CrossRefGoogle Scholar
  98. 98.
    Vestal DJ, Ranscht B (1992) J Cell Biol 119:451–461CrossRefGoogle Scholar
  99. 99.
    Virla EG, Casuso M, Frias EA (2010) Crop protection 29:635–638CrossRefGoogle Scholar
  100. 100.
    Walters FS, Mullin CA (1988) Arch Insect Biochem Physiol 9:35–46CrossRefGoogle Scholar
  101. 101.
    Wang P, Zhang X, Zhang J (2005) Insect Biochem Mol Biol 35:611–620CrossRefGoogle Scholar
  102. 102.
    Warren JT, Petryk A, Marques G, Parvy JP, Shinoda T, Itoyama K, Kobayashi J, Jarcho M, Li Y, O’Connor MB, Dauphin-Villemant C, Gilbert LI (2004) Insect Biochem Mol Biol 34:991–1010CrossRefGoogle Scholar
  103. 103.
    Wilkinson TL, Ashford DA, Pritchard J, Douglas AE (1997) J Exp Biol 200:2137–2143Google Scholar
  104. 104.
    Wolfersberger MG (1984) Am Zool 24:187–197Google Scholar
  105. 105.
    Yarasi B, Sadumpati V, Immanni CP, Vudem DR, Khareedu VR (2008) BMC Plant Biol 8:102CrossRefGoogle Scholar
  106. 106.
    Young MJ, Filichkin SA (1999) Trends Microbiol 7:346–347CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.CSIR-National Botanical Research InstituteCouncil of Scientific and Industrial ResearchLucknowIndia

Personalised recommendations