The Protein Journal

, Volume 31, Issue 2, pp 137–140

Effects of Free Ca2+ on Kinetic Characteristics of Holotransketolase

  • Olga N. Solovjeva
  • Irina A. Sevostyanova
  • Vladimir A. Yurshev
  • Vitalii A. Selivanov
  • German A. Kochetov
Article
  • 74 Downloads

Abstract

Catalytic activity has been demonstrated for holotransketolase in the absence of free bivalent cations in the medium. The two active centers of the enzyme are equivalent in both the catalytic activity and the affinity for the substrates. In the presence of free Ca2+ (added to the medium from an external source), this equivalence is lost: negative cooperativity is induced on binding of either xylulose 5-phosphate (donor substrate) or ribose 5-phosphate (acceptor substrate), whereupon the catalytic conversion of the bound substrates causes the interaction between the centers to become positively cooperative. Moreover, the enzyme total activity increase is observed.

Keywords

Calcium ions Kinetic parameters Negative cooperativity Positively cooperative Transketolase 

Abbreviations

TK

Transketolase

TDP

Thiamine diphosphate

R5P

Ribose 5-phosphate

X5P

Xylulose 5-phosphate

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Olga N. Solovjeva
    • 1
  • Irina A. Sevostyanova
    • 1
  • Vladimir A. Yurshev
    • 1
  • Vitalii A. Selivanov
    • 1
  • German A. Kochetov
    • 1
  1. 1.A.N. Belozersky Institute of Physico-chemical BiologyMoscow State UniversityMoscowRussia

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