The Protein Journal

, Volume 30, Issue 2, pp 138–142 | Cite as

Proteomic Analysis of Differentially Expressed Proteins between Stenotic and Normal Colon Segment Tissues Derived from Patients with Hirschsprung’s Disease

  • Hong Gao
  • Xiaojing He
  • Mei Wu
  • Zhibo Zhang
  • Dajia Wang
  • Liangying Lv
  • Zhenwei Su
  • Ying Huang
Article
First Online:

Abstract

Hirschsprung’s disease (HSCR) is the most common identifiable developmental disorder of the enteric nervous system. The present study was designed to analyze the differential proteomic patterns in stenotic colon segment tissues from patients with HSCR. We analyzed 20 paired stenotic and normal colon segment tissues from patients with HSCR, and identified 13 proteins from stenotic segment tissues peptide fingerprint mapping and SELDI MS that were separated using 2-DE. The protein levels of four selected proteins (α-actinin-4, ACTN4; myosin regulatory light chain interacting protein, MYLIP; fatty acid binding protein 7, FABP7; bone morphogenetic protein receptor type 1A, BMPR1A) were further validated by Western blot analysis. This study, investigating for the first time proteomic changes in stenotic colon segment tissues from patients with HSCR, provides potential markers or promising new candidate actors for the pathogenesis of HSCR.

Keywords

Hirschsprung’s disease Proteomic analysis Biomarkers Stenotic colon segment tissues 

Abbreviations

HSCR

Hirschsprung’s disease

2-DE

Two-dimensional electrophoresis

MALDI-TOF-MS

Matrix assisted laser desorption ionisation time-of-flight mass spectrometry

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Notes

Acknowledgments

This work was supported by grant from the National Natural Science Foundation of China (No. 30772277). The authors are grateful to Prof. Weilin Wang and Prof. Lianying Wang of the Department of Pediatric Surgery, Shengjing Hospital Affiliated to China Medical University for their expert technical assistance.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hong Gao
    • 1
  • Xiaojing He
    • 2
  • Mei Wu
    • 1
  • Zhibo Zhang
    • 3
  • Dajia Wang
    • 3
  • Liangying Lv
    • 3
  • Zhenwei Su
    • 4
  • Ying Huang
    • 3
  1. 1.Key Laboratory of Health Ministry for Congenital MalformationsShengjing Hospital Affiliated to China Medical UniversityShenyang, Liaoning ProvinceChina
  2. 2.Department of PharmacologyShengjing Hospital Affiliated to China Medical UniversityShenyang, Liaoning ProvinceChina
  3. 3.Department of Pediatric SurgeryShengjing Hospital Affiliated to China Medical UniversityShenyang, Liaoning ProvinceChina
  4. 4.Department of Pediatric SurgeryWomen and Children’s HospitalDandong, Liaoning ProvinceChina

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