The Protein Journal

, Volume 27, Issue 6, pp 371–375

Isolation and Characterization of Low-Molecular-Weight Chromium-binding Substance (LMWCr) from Chicken Liver

Article

Abstract

Chromodulin (also known as low-molecular-weight chromium-binding substance, LMWCr) is a chromium-binding oligopeptide proposed to play a role in insulin signaling and chromium transport in mammals. This laboratory has isolated and purified this material from a non-mammalian source, an avian. Spectroscopic and physical characterization of the isolated material suggests the material is an oligopeptide with a multinuclear chromium assembly bridged via asparatate and glutamate residues very similar to its mammalian counterparts. The isolated material also possesses a biological activity similar to other LMWCr isolates.

Keywords

Chromium Chicken Low-molecular-weight chromium-binding substance Chromodulin 

Abbreviations

LMWCr

Low-molecular-weight chromium-binding substance

ADAM 19

A disintegrin and metalloproteinase domain 19

Glu

Glutamate

Asp

Aspartate

mL

Milliliter

M

Molar

mM

Millimolar

μM

Micomolar

g

Gravity

ºC

Degree celsius

%

Percent

pH

Measure of acidity or basicity

cm

Centimeter

nm

Nanometer

μL

Microliter

ppm

Parts per million

DEAE

(Diethylaminoethyl) cellulose

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

1H NMR

Proton nuclear magnetic resonance

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of ChemistryGeorgia Southern UniversityStatesboroUSA

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