The Protein Journal

, Volume 27, Issue 5, pp 309–318 | Cite as

Identification of Critical Amino Acid Residues for Human iNOS Functional Activity

  • Saule Naureckiene
  • Sreekumar R. Kodangattil
  • Edward J. Kaftan
  • Philip G. Jones
  • Jeffrey D. Kennedy
  • Kathryn E. Rogers
  • Pranab K. Chanda
Article

Abstract

Nitric oxide (NO) is a short-lived signaling molecule that mediates a variety of biological functions, including vascular homeostasis, neurotransmission, antimicrobial defense and antitumor activities. Three known NOS isoforms (eNOS, nNOS and iNOS) have been cloned and sequenced. Here, we show that upon expression in Escherichia coli using a novel expression vector, an iNOS sequence containing three mutations (A805D, F831S and L832P) within the iNOS reductase domain produced very little functionally active iNOS protein compared to the wild type (wt) iNOS. Each of these point mutations also was individually constructed into the wt iNOS sequence. The activity of the iNOS protein containing the A805D mutation was comparable to wt, while a drastic reduction in iNOS activity was observed for the F831S and L832P mutants. A comparison of the molecular models of the reductase domain of the wt and mutant iNOS revealed a reduced core packing density for the F831S and L832P mutations compared to wt. In addition, the modeling also suggests altered hydrogen bonding, van der Waals and hydrophobic interactions of these mutants.

Keywords

Nitric oxide synthase Nitric oxide reductase Lac-tac-tac promoter Point mutation E. coli expression 

Abbreviations

NO

Nitric oxide

NOS

Nitric oxide synthase

LPS

Lipopolysaccharide

FAD

Flavin Adenine Dinucleotide

H4B

Tetrahydrobiopterin

SMT

2-Methyl-2-thiopseudourea, sulfate

PBITU

[S, S′-1,3-Phenylene-bis (1,2-ethanediyl)-bis-isothiourea, 2HBr]

l-NIL

[l-N6-(1-Iminoethyl)lysine, DiHCl]

1400W

[N-(3-Aminomethyl)benzylacetamidine, 2HCl]

l-NNA

NG-Nitro-l-arginine

7-Ni

7-Nitroindazole

N-PLA

NG-Propyl-l-arginine

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Saule Naureckiene
    • 1
    • 2
  • Sreekumar R. Kodangattil
    • 3
  • Edward J. Kaftan
    • 1
  • Philip G. Jones
    • 1
    • 4
  • Jeffrey D. Kennedy
    • 1
  • Kathryn E. Rogers
    • 1
    • 5
  • Pranab K. Chanda
    • 1
  1. 1.Neuroscience Discovery ResearchWyeth ResearchPrincetonUSA
  2. 2.J&J PRD, LLCTitusvilleUSA
  3. 3.Biological TechnologiesWyeth ResearchPrincetonUSA
  4. 4.Sepracor Inc.MarlboroughUSA
  5. 5.Envivo PharmaceuticalsWatertownUSA

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