The Protein Journal

, Volume 25, Issue 7–8, pp 492–502 | Cite as

Structural and Functional Properties of Cr 5, a New Lys49 Phospholipase A2 Homologue Isolated from the Venom of the Snake Calloselasma rhodostoma

  • V. L. Bonfim
  • L. A. Ponce-Soto
  • J. C. Novello
  • S. Marangoni
Article

Cr 5 PLA2 homologous (K49) was isolated from Calloselasma rhodostoma venom in only one chromatographic step in reverse phase HPLC (RP-HPLC) (on μ-Bondapack C-18). A molecular mass of 13.965 Da was determined by MALDI-TOF mass spectrometry. The amino acid composition showed that Cr 5 had a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical residues of a basic PLA2. The complete amino acid sequence of Cr 5 PLA2 contains 120 residues, resulting in a calculated pI value of 5.55. This sequence shows high identity values when compared to other K49 PLA2s isolated from the venoms of viperid snakes. Lower identity is observed in comparison to D49 PLA2s. The sequence found was SLVELGKMIL QETGKNPAKS YGAYGCNCGV LGRHKPKDAT DRCCFVHKCC YKKLTGCDPK KDRYSYSWKD KTIVCGENNP CLKEMCECDK AVAICLRENL DTYNKKYRYL KPFCKKADDC. In mice, Cr 5 induced myonecrosis and edema upon intramuscular and intravenous injections, respectively. The LD50 of Cr 5 was 0.070 mg/kg of the animal weight, by intracerebroventricular (i.c.v.) route. In vitro, the toxin caused rapid cytolytic effect upon mouse skeletal muscle myoblasts in culture. The isolation of this PLA2 and the combined structural and functional information obtained classify Cr 5 as a new member of the K49 PLA2 family, since it presents typical features from such proteins.

Keywords

Phospholipase A2, Lys49 myotoxin snake venom Calloselasma rhodostoma 

Notes

Acknowledgments

The authors thank Mr. Paulo A. Baldasso for general technical help. This work was supported by CAPES and FAPESP and is part of a PhD thesis by Vera Lucia Bonfim.

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Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • V. L. Bonfim
    • 1
  • L. A. Ponce-Soto
    • 1
  • J. C. Novello
    • 1
  • S. Marangoni
    • 1
  1. 1.Department of Biochemistry, Institute of BiologyState University of CampinasCampinasBrazil

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