The Protein Journal

, Volume 24, Issue 6, pp 369–377 | Cite as

Single Step Purification, Characterization and N-Terminal Sequences of a Mannose Specific Lectin from Mulberry Seeds

  • Nurul Absar
  • T. Yeasmin
  • M. Salim Raza
  • S. Kumar Sarkar
  • F. Arisaka
Article

Abstract

A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose. The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the lectin is also discussed.

Keywords

Biological activity mulberry seed mannose specific lectin N-terminal sequences 

Abbreviations

Con A

Concanavalin A

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

MW

Molecular weight

DEAE

diethylaminoethyl

CM

Carboxy methyl

BSA

Bovine serum albumin

TLC

Thin layer chromatography

ML

Mulberry lectin

A. int.

Artocarpus integrifolia

C. cren.

Castanea crenata

O. sat.

Oryza sative.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Nurul Absar
    • 1
  • T. Yeasmin
    • 1
  • M. Salim Raza
    • 1
  • S. Kumar Sarkar
    • 2
  • F. Arisaka
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of RajshahiRajshahi
  2. 2.Department of Bimolecular EngineeringFaculty of Bioscience and Biotechnology, T I TYokohamaJapan

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