Single Step Purification, Characterization and N-Terminal Sequences of a Mannose Specific Lectin from Mulberry Seeds
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Abstract
A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose. The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the lectin is also discussed.
Keywords
Biological activity mulberry seed mannose specific lectin N-terminal sequencesAbbreviations
- Con A
Concanavalin A
- SDS-PAGE
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
- MW
Molecular weight
- DEAE
diethylaminoethyl
- CM
Carboxy methyl
- BSA
Bovine serum albumin
- TLC
Thin layer chromatography
- ML
Mulberry lectin
- A. int.
Artocarpus integrifolia
- C. cren.
Castanea crenata
- O. sat.
Oryza sative.
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