Analysis of Hybrid Sorubim Protein Films Incorporated with Glycerol and Clove Essential Oil for Packaging Applications

  • Rosiane de Souza Silva
  • Barbara Matias Moreira Santos
  • Gustavo Graciano FonsecaEmail author
  • Carlos Prentice
  • William Renzo Cortez-Vega
Original Paper


The indiscriminate disposal and the accumulation of non-biodegradable packaging have been causing serious environmental problems. One of the alternatives is the use of biodegradable films. Proteins are among the natural biopolymers most used to produce biofilms. This study aimed to produce and characterize biofilms obtained from hybrid sorubim (Pseudoplatystoma reticulatum × Pseudoplatystoma corruscans) protein isolate (HSPI) incorporated with glycerol (G) and clove essential oil (CEO). The HSPI was obtained by the pH shift method, using as raw material the mechanically separated meat of hybrid sorubim. The films presented different properties depending on the concentrations of the studied variables. In general, the film with the best characteristics was the treatment 6 (25% G, 2.5 g HSPI and 0.5 g CEO). Due to the low concentration of CEO used, the films were not able to efficiently inhibit microbial activity.

Graphic Abstract


Polymers Fish protein isolate Essential oil 



The authors gratefully acknowledge the Brazilian research funding agencies CNPq, CAPES and FUNDECT for the financial support.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Bioengineering, Faculty of Biological and Environmental SciencesFederal University of Grande DouradosDouradosBrazil
  2. 2.Laboratory of Food Technology, School of Food and ChemistryFederal University of Rio GrandeRio GrandeBrazil

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