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Journal of Polymers and the Environment

, Volume 27, Issue 12, pp 2793–2803 | Cite as

Biodegradation of Cellulose in Laboratory-Scale Bioreactors: Experimental and Numerical Studies

  • Antonis MistriotisEmail author
  • Nikoleta-Georgia Papardaki
  • Astero Provata
Original paper
  • 38 Downloads

Abstract

Standard tests such as ISO 17556 (Plastics - determination of the ultimate aerobic biodegradability in soil by measuring the oxygen demand in a respirometer or the amount of carbon dioxide evolved. International Organization for Standardization, Geneva, Switzerland, 2019) or ASTM D5988 (Standard test method for determining aerobic biodegradation of plastic materials in soil. ASTM International, West Conshohocken, 2018) have been developed for measuring the biodegradation of polymers and assessing their biodegradability in soil. In several experiments performed according to these standard tests, the measured biodegradation of cellulose was reported unexpectedly between 80 and 85%. These results are difficult to justify since cellulose is a well-known biodegradable material. In the present study, this phenomenon is explained as the consequence of starvation occurring in a confined bioreactor. It is proposed that the favourable conditions applied to the small-size bioreactors accelerate the proliferation of the microorganisms which are responsible for the biodegradation. Such a dense microbial population may face starvation when cellulose is consumed. It is assumed that starvation causes the secretion of an inhibitory chemical signal, which can diffuse to neighbouring sites still containing food, and suppresses biodegradation. This hypothesis was supported by two experiments. First, it was shown that the final measured biodegradation increased when the microbial growth rate decreased by reducing temperature. In the second experiment, it was shown that the biodegradation proceeded slower when a new cellulose quantity was inserted into a previously used soil substrate. To confirm the hypothesis regarding the presence of an inhibitory starvation factor, which can suppress biodegradation, a Kinetic Monte Carlo (KMC) model was also developed. The KMC simulations reproduced qualitatively the experimental findings.

Keywords

Biodegradation Cellulose Starvation of microorganisms Standard tests for biodegradation Small-scale bioreactors Kinetic Monte Carlo simulations 

Notes

Acknowledgements

The authors would like to thank Professor D. Briassoulis for his support and for many fruitful discussions. This work was supported by computational time granted from the Greek Research & Technology Network (GRNET) in the National HPC facility–ARIS, under Project ID: PR007011.

Supplementary material

10924_2019_1560_MOESM1_ESM.xlsx (23 kb)
Supplementary material 1 (XLSX 23 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Natural Resources and Agricultural EngineeringAgricultural University of AthensAthensGreece
  2. 2.Institute of Nanoscience and NanotechnologyNational Center for Scientific Research “Demokritos”AthensGreece

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