Journal of Polymers and the Environment

, Volume 27, Issue 12, pp 2804–2818 | Cite as

Scleroglucan Production by Sclerotium rolfsii ATCC 201126 from Amylaceous and Sugarcane Molasses-Based Media: Promising Insights for Sustainable and Ecofriendly Scaling-Up

  • Alejandra Leonor Valdez
  • Jaime Daniel Babot
  • Jochen Schmid
  • Osvaldo Daniel Delgado
  • Julia Inés FariñaEmail author
Original paper


Scleroglucan is a β-glucan exopolysaccharide (EPS) efficiently produced by Sclerotium rolfsii ATCC 201126, with attractive properties for a wide range of industries. Its production was herein comparatively assessed with nine alternative C- and twelve N-sources. When comparing conventional sucrose-based Production Medium PM20 (8.41 g C/L + NaNO3 as N-source) at shake-flask-scale vs. alternative C-source versions, soluble starch and sugarcane molasses led to efficient EPS production. At bioreactor scale, starch-based medium led to highest EPS production (7.95 g/L), recovery (~ 52%) and productivities (0.11 g EPS/L h; 0.018 g EPS/gbiomass h). Molasses, though leading to lower EPS production (5.11 g/L), could be envisaged as a promising agroindustrial sub-product for adding value and innovation. Oxalate side-product varied with C- and N-sources, with no clear detrimental relationship with EPS production. Agroindustrial sub-products showed then to be suitable as alternative substrates for efficient, low-cost, and scalable EPS production, thus opening new perspectives for medium reformulation and sustainable EPS production.


Sclerotium rolfsii Scleroglucan Corn starch Sugarcane molasses Sustainable production 



The study was financially supported by the National Council for Scientific and Technical Research (CONICET) (Grant PIP0976), the National University of Catamarca (UNCa) (Grant PIO-UNCa 0100054), and the National Agency for the Promotion of Science and Technology (ANPCyT) along with the Deutsche Forschungsgemeinschaft (DFG) (Bilateral Cooperation project CONICET-MINCyT-DFG level II). Authors gratefully acknowledge Lic. María Florencia Ladetto for critical analyses of FT-IR spectra.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Authors and Affiliations

  1. 1.Mycodiversity & Mycoprospection LabPlanta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICETSM TucumánArgentina
  2. 2.Centro de Referencia para Lactobacilos, CERELA-CONICETSM TucumánArgentina
  3. 3.Campus Straubing for Biotechnology and SustainabilityTechnical University of MunichStraubingGermany
  4. 4.Department of Biotechnology and Food ScienceNorwegian University of Science and TechnologyTrondheimNorway
  5. 5.Faculty of Exact and Natural SciencesNational University of CatamarcaCatamarcaArgentina

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