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Journal of Polymers and the Environment

, Volume 27, Issue 5, pp 1105–1111 | Cite as

Physico-chemical Characterization of Poly(3-Hydroxybutyrate) Produced by Halomonas salina, Isolated from a Hypersaline Microbial Mat

  • Emanuel Hernández-Núñez
  • Carolina Alejandra Martínez-Gutiérrez
  • Alejandro López-Cortés
  • Ma. Leopoldina Aguirre-Macedo
  • Carolina Tabasco-Novelo
  • Maria Ortencia González-DíazEmail author
  • José Q. García-MaldonadoEmail author
Original paper
  • 109 Downloads

Abstract

In this work, the characterization of poly(3-hydroxybutyrate) PHB produced by Halomonas salina isolated from a hypersaline microbial mat from Guerrero Negro, Baja California Sur, Mexico, is reported. The bacterial strain was able to produce isotactic PHB biopolymer with glucose (1%) as a single carbon source. The chemical structure of the polymer obtained was confirmed by Fourier-transform infrared (FTIR) and Nuclear magnetic resonance (NMR) spectroscopy. The polymer was thermally stable up to 225 °C and Differential scanning calorimetry (DSC) analysis showed a melting temperature (Tm) of 173.6 °C. The obtained polymer presented a lower degree of crystallinity (39.3%) in comparison with PHB produced by other bacteria and polyhydroxyalkanoate co-polymers. Thus, the PHB biopolymer obtained in this study, could be recognized as more suitable for practical use, contributing to the repertoire of available bioplastics for further potential biotechnological applications, in which elastic polymers are needed.

Keywords

Poly(3-hydroxybutyrate) Halomonas salina Biopolymer Hypersaline microbial mats 

Notes

Acknowledgements

We thank to LANNBIO CINVESTAV-Merida (projects FOMIX-Yucatan 2008-108160, CONACYT LAB-2009-01 No. 123913 and CB20121 178947) for the TGA, XRD, NMR and SEM-EDX measurements. The authors acknowledge the Cátedras CONACYT projects 3139 and 1568. We highly appreciate the technical support of Hever Latisnere-Barragán in the laboratory (CIBNOR) and Dr Wilberth Herrera Kao for assistance with GPC measurements.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Emanuel Hernández-Núñez
    • 1
  • Carolina Alejandra Martínez-Gutiérrez
    • 2
  • Alejandro López-Cortés
    • 2
  • Ma. Leopoldina Aguirre-Macedo
    • 3
  • Carolina Tabasco-Novelo
    • 3
  • Maria Ortencia González-Díaz
    • 4
    Email author
  • José Q. García-Maldonado
    • 1
    Email author
  1. 1.CONACYT - Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalDepartamento de Recursos del MarMéridaMexico
  2. 2.Centro de Investigaciones Biológicas del Noroeste S.CLa PazMexico
  3. 3.Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalUnidad MéridaMéridaMexico
  4. 4.CONACYT - Centro de Investigación Científica de Yucatán A. CMéridaMexico

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