Journal of Polymers and the Environment

, Volume 16, Issue 2, pp 141–146 | Cite as

Mechanism and Characterization of Polyamide 4 Degradation by Pseudomonas sp.

  • Naoko Yamano
  • Atsuyoshi Nakayama
  • Norioki Kawasaki
  • Noboru Yamamoto
  • Seiichi Aiba
Original Paper


We identified a biodegrading microorganism of polyamide (nylon) 4, a linear polymer of γ-aminobutyric acid (GABA). From activated sludge, the biodegrading bacteria strains of Pseudomonas sp. were isolated and identified by their taxonomic characteristics and nucleotide sequences of 16S rDNA. One strain, ND-11, was grown on a minimal medium containing polyamide 4 (PA4) as the sole carbon source. The strain produced GABA as a degradation intermediate, as identified by analyzing the NMR spectra of degraded products. The culture supernatant of strain ND-11 degraded the emulsified PA4 completely within one day. These results suggest that the ND-11 strain degraded PA4 using its extracellular enzymes to hydrolyze amide bonds.


Polyamide Nylon Biodegradation Degrading bacteria Activated sludge γ-Aminobutyric acid 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Naoko Yamano
    • 1
  • Atsuyoshi Nakayama
    • 1
  • Norioki Kawasaki
    • 1
  • Noboru Yamamoto
    • 1
  • Seiichi Aiba
    • 1
  1. 1.Research Institute for Innovation in Sustainable ChemistryNational Institute of Advanced Industrial Science and Technology (AIST)IkedaJapan

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