Mechanism and Characterization of Polyamide 4 Degradation by Pseudomonas sp.
We identified a biodegrading microorganism of polyamide (nylon) 4, a linear polymer of γ-aminobutyric acid (GABA). From activated sludge, the biodegrading bacteria strains of Pseudomonas sp. were isolated and identified by their taxonomic characteristics and nucleotide sequences of 16S rDNA. One strain, ND-11, was grown on a minimal medium containing polyamide 4 (PA4) as the sole carbon source. The strain produced GABA as a degradation intermediate, as identified by analyzing the NMR spectra of degraded products. The culture supernatant of strain ND-11 degraded the emulsified PA4 completely within one day. These results suggest that the ND-11 strain degraded PA4 using its extracellular enzymes to hydrolyze amide bonds.
KeywordsPolyamide Nylon Biodegradation Degrading bacteria Activated sludge γ-Aminobutyric acid
- 1.Negoro S, Shinagawa H, Nakata A, Kinoshita S, Hatozaki T, Okada H (1980) J Bacteriol 143:238–245Google Scholar
- 2.Negoro S, Taniguchi T, Kanaoka M, Kimura H, Okada H (1983) J Bacteriol 155:22–31Google Scholar
- 3.Kakudo S, Negoro S, Urabe I, Okada H (1993) Appl Environ Microbiol 59:3978–3980Google Scholar
- 4.Deguchi T, Kakezawa M, Nishida T (1997) Appl Environ Microbiol 63:329–331Google Scholar
- 5.Deguchi T, Kitaoka Y, Kakezawa M, Nishida T (1998) Appl Environ Microbiol 64:1366–1371Google Scholar
- 8.Higuchi T, Hayashi H, Abe K (1997) J Bacteriol 179:3362–3364Google Scholar
- 9.Mountfort DO, Pybus V (1992) Appl Environ Microbiol 58:237–242Google Scholar
- 11.Secades P, Guijarro J (1999) Appl Environ Microbiol 65:3969–3975Google Scholar