Journal of Polymers and the Environment

, Volume 16, Issue 2, pp 141–146 | Cite as

Mechanism and Characterization of Polyamide 4 Degradation by Pseudomonas sp.

  • Naoko Yamano
  • Atsuyoshi Nakayama
  • Norioki Kawasaki
  • Noboru Yamamoto
  • Seiichi Aiba
Original Paper

Abstract

We identified a biodegrading microorganism of polyamide (nylon) 4, a linear polymer of γ-aminobutyric acid (GABA). From activated sludge, the biodegrading bacteria strains of Pseudomonas sp. were isolated and identified by their taxonomic characteristics and nucleotide sequences of 16S rDNA. One strain, ND-11, was grown on a minimal medium containing polyamide 4 (PA4) as the sole carbon source. The strain produced GABA as a degradation intermediate, as identified by analyzing the NMR spectra of degraded products. The culture supernatant of strain ND-11 degraded the emulsified PA4 completely within one day. These results suggest that the ND-11 strain degraded PA4 using its extracellular enzymes to hydrolyze amide bonds.

Keywords

Polyamide Nylon Biodegradation Degrading bacteria Activated sludge γ-Aminobutyric acid 

References

  1. 1.
    Negoro S, Shinagawa H, Nakata A, Kinoshita S, Hatozaki T, Okada H (1980) J Bacteriol 143:238–245Google Scholar
  2. 2.
    Negoro S, Taniguchi T, Kanaoka M, Kimura H, Okada H (1983) J Bacteriol 155:22–31Google Scholar
  3. 3.
    Kakudo S, Negoro S, Urabe I, Okada H (1993) Appl Environ Microbiol 59:3978–3980Google Scholar
  4. 4.
    Deguchi T, Kakezawa M, Nishida T (1997) Appl Environ Microbiol 63:329–331Google Scholar
  5. 5.
    Deguchi T, Kitaoka Y, Kakezawa M, Nishida T (1998) Appl Environ Microbiol 64:1366–1371Google Scholar
  6. 6.
    Kawasaki N, Nakayama A, Yamano N, Takeda S, Kawata Y, Yamamoto N, Aiba S (2005) Polymer 46:9987–9993CrossRefGoogle Scholar
  7. 7.
    Hashimoto K, Hamano T, Okada M (1994) J Appl Polymer Sci 54:1579–1583CrossRefGoogle Scholar
  8. 8.
    Higuchi T, Hayashi H, Abe K (1997) J Bacteriol 179:3362–3364Google Scholar
  9. 9.
    Mountfort DO, Pybus V (1992) Appl Environ Microbiol 58:237–242Google Scholar
  10. 10.
    Nishida H, Tokiwa Y (1993) J Environ Polym Degrad 1:227–233CrossRefGoogle Scholar
  11. 11.
    Secades P, Guijarro J (1999) Appl Environ Microbiol 65:3969–3975Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Naoko Yamano
    • 1
  • Atsuyoshi Nakayama
    • 1
  • Norioki Kawasaki
    • 1
  • Noboru Yamamoto
    • 1
  • Seiichi Aiba
    • 1
  1. 1.Research Institute for Innovation in Sustainable ChemistryNational Institute of Advanced Industrial Science and Technology (AIST)IkedaJapan

Personalised recommendations