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Magnetic Resonance Imaging of the Brains of Three Peramelemorphian Marsupials

  • Yamila GurovichEmail author
  • Andre Bongers
  • Ken W. S. Ashwell
Original Paper

Abstract

Peramelemorphians (bandicoots and bilbies) are a unique and diverse group of digging Australasian marsupials, but their behavioral neurology and neuroanatomy is poorly known. Here, we have used Magnetic Resonance Imaging (MRI) to study the brains of three peramelemorphians: two bandicoots (Perameles nasuta and Isoodon obesulus) and the bilby (Macrotis lagotis), which is endangered. These brains had been stored in formaldehyde solution for more than 80 years and one of our goals was to demonstrate the feasibilty of extracting detailed comparative neuroanatomical information from the long-term preserved brains of rare, endangered, and extinct animals. High resolution anatomical and Diffusion Tensor Imaging was performed using a 9.4-T Bruker BioSpec 94/20 Avance III MRI system (Bruker, Ettlingen, Germany) located at the UNSW in Sydney. We were able to differentiate areal and laminar topography within isocortical areas (primary somatosensory – S1; and visual - V1, V2), as well as subdivisions within olfactory and limbic allocortical regions (cingulate, hippocampal). Resolution of subcortical structures was sufficient to differentiate α and β segments within the visual nucleus of the thalamus. We identified several previously unrecognized longitudinal association fiber systems as well as a rich array of sensory thalamocortical connections. Dense fiber pathways were observed to S1 and in the midbrain auditory pathways (mainly the lateral lemniscus, but also the brachium of the inferior colliculus). Our findings demonstrate the feasibility of using this sort of imaging of archived brains to analyze the neuroanatomy of rare and evolutionarily significant species and to relate these findings to the behavioral neurology of those species.

Keywords

Bandicoot Bilby Peramelemorphia MRI Brain Neuroanatomy 

Notes

Acknowledgments

We would like to thank Dr. Sandy Ingleby of the Australian Musuem for the loan of the peramelid and bilby brains. The authors would also like to acknowledge Dr. Craig Hardman (UNSW) for his help with formatting the original data and for his helpful discussion on this topic.

The facilities, and the scientific and technical assistance of the National Imaging Facility at the UNSW Mark Wainwright Analytical Centre, Biological Imaging Resources Laboratory MRI facility are gratefully acknowledged.

Emeritus Professor John Nelson of Monash University and Dr. Leo Joseph of CSIRO kindly gave us permission to photograph sectioned and stained peramelid brains from the Nelson Brain Collection in Canberra.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.CIEMEP, CONICET-UNPSJBEsquelArgentina
  2. 2.Department of Anatomy, School of Medical SciencesThe University of New South WalesSydneyAustralia
  3. 3.Biological Resources and Imaging LaboratoryThe University of New South WalesSydneyAustralia

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