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Journal of Mathematical Chemistry

, Volume 56, Issue 8, pp 2525–2536 | Cite as

The structural modeling of EF-hand motifs in parvalbumin

  • Yun Zhao
  • Jianfeng He
  • Jing Li
Original Paper

Abstract

Parvalbumin (Parv) is a typical protein with EF-hand motifs that play an important role in many physiological processes. We present a novel free energy to model the skeletal C\(_\alpha \) chain of the protein from the basic principle of mathematics and physics. Starting from the crystal structure of Parv (PDB code 2PVB), we first analyze the profile of the C\(_\alpha \) bond and torsion angles over the segment that contains the secondary structures. Then the parameters in the energy function are evaluated for the helix ABCD fragment that contains two EF-hand domains in Parv. Meanwhile an eight-soliton configuration at the energy minimum is constructed to model the conformation of ABCD fragment. The deviation of the conformation constructed from the model away from the crystal structure is as small as 1.28 Å. The structural modeling stems from the physical energy, which is a benefit relative to the statistics-based or knowledge-based technologies.

Keywords

Parvalbumin EF-hand Protein structural modeling Free energy Soliton 

Notes

Acknowledgements

We would like to thank Prof. Antti J. Niemi of Uppsala University, our cooperator in biophysics, for continued discussions on the theory and method. We also thanks the support of the international cooperation project of Beijing Institute of Technology.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of PhysicsBeijing Institute of TechnologyBeijingPeople’s Republic of China
  2. 2.Beijing Genetech Pharmaceutical Co. Ltd.BeijingPeople’s Republic of China

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