Journal of Mathematical Chemistry

, Volume 49, Issue 9, pp 1949–1960 | Cite as

Enzyme kinetics with a twist

Original Paper

Abstract

A different approach to enzyme kinetics stressing the cyclic nature of the catalytic process is presented. The time-dependence of the substrate concentration is derived in a simple way not invoking the quasi-steady-state approximation. According to this approach the turnover rate can be written as the ratio of two parameters with a direct meaning: enzyme efficiency and average cycle duration. Real kinetic data for two enzyme-substrate pairs is used to show that the enzyme kinetic efficiency is best measured by the turnover rate.

Keywords

Enzyme kinetics Quasi-steady-state approximation Lambert function 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Centro de Química-Física Molecular and IN–Institute of Nanoscience and Nanotechnology, Instituto Superior TécnicoUniversidade Técnica de LisboaLisboaPortugal

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