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Journal of Mathematical Chemistry

, Volume 42, Issue 4, pp 789–813 | Cite as

An alternative analysis of enzyme systems based on the whole reaction time: evaluation of the kinetic parameters and initial enzyme concentration

  • R. Varón
  • M. Garcia-Moreno
  • J. Masiá-Pérez
  • F. García-Molina
  • F. García-Cánovas
  • E. Arias
  • E. Arribas
  • F. García-Sevilla
Article
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Abstract

This work presents an alternative analysis of the integrated rate equations corresponding to the simple Michaelis-Menten mechanism without product inhibition. The suggested new results are reached under a minimal set of assumptions and include, as a particular case, the classical integrated Michaelis–Menten equation. Experimental designs and a kinetic data analysis are suggested to the estimation of the maximum steady-state rate, V max, the Michaelis–Menten constant, K m, the initial enzyme concentration, [E]0, and the catalytic constant, k 2. The goodness of the analysis is tested with simulated time progress curves obtained by numerical integration.

Keywords

Enzyme kinetics Michaelis–Menten integrated equation product rate substrate rate numerical integration 
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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • R. Varón
    • 1
  • M. Garcia-Moreno
    • 1
  • J. Masiá-Pérez
    • 2
  • F. García-Molina
    • 3
  • F. García-Cánovas
    • 3
  • E. Arias
    • 4
  • E. Arribas
    • 5
  • F. García-Sevilla
    • 6
  1. 1.Departamento de Química Física, Escuela Politécnica SuperiorUniversidad de Castilla-La ManchaAlbaceteSpain
  2. 2.Servicio de Cardiología, Complejo HospitalarioUniversitario de AlbaceteAlbaceteSpain
  3. 3.Departamento de Bioquímica y Biología Molecular A, Facultad de BiologíaUniversidad de MurciaMurciaSpain
  4. 4.Departamento de Sistemas Informáticos, Escuela Politécnica Superior de AlbaceteUniversidad de Castilla-La ManchaAlbaceteSpain
  5. 5.Departamento de Física Aplicada, Escuela Politécnica Superior de AlbaceteUniversidad de Castilla-La ManchaAlbaceteSpain
  6. 6.Departamento de Ingeniería Electrónica, Eléctrica, Automática y Comunicaciones, Escuela Politécnica Superior de AlbaceteUniversidad de Castilla-La ManchaAlbaceteSpain

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