Journal of Insect Behavior

, Volume 30, Issue 2, pp 170–179 | Cite as

Strategic Investment in Sperm Removal Behaviour in a Bushcricket

  • Maike Foraita
  • Sophie Lehfeldt
  • Klaus Reinhold
  • Steven A. Ramm
Article

Abstract

Multiple mating by females is widespread and generates sperm competition among the ejaculates of rival males over fertilization. One way in which males can avoid or reduce sperm competition is by displacing or removing previous males’ sperm from female sperm stores. An apparent example of this occurs in the bushcricket Metaplastes ornatus. Males perform a specialised sperm removal behaviour (SRB), using their highly-derived subgenital plate, with which they remove sperm from the female’s spermatheca during the early phases of mating before transferring a spermatophore of their own. Here we investigated whether males strategically invest in SRB according to the amount of previously stored sperm present in females. Each male was tested twice, once with a female containing sperm (‘filled’ condition) and once with a female from whom most previously deposited sperm had recently been removed by another male (‘emptied’ condition). For comparison, a separate group of males was paired with virgin females. Males did not discriminate between non-virgin females in the ‘emptied’ or ‘filled’ conditions in terms of their investment in SRB, suggesting they may not able to perceive the amount of sperm present in the female’s spermatheca. By contrast, male investment in SRB was significantly reduced in pairings with virgin females, indicating that males are sensitive to some aspect of a female’s mating status. Our results thus suggest that males modulate SRB in response to female-mediated cues, possibly chemical cues left by previous males, which would not be present on virgin but would be on non-virgin females.

Keywords

Insect reproduction sperm competition bush cricket cryptic mate choice 

Supplementary material

10905_2017_9608_MOESM1_ESM.docx (28 kb)
ESM 1Supplementary Material Table 1 (DOCX 27 kb)
ESM 2

(MP4 201147 kb)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Evolutionary BiologyBielefeld UniversityBielefeldGermany
  2. 2.School of Biological, Earth and Environmental SciencesUniversity College CorkCorkIreland

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