Journal of Insect Behavior

, Volume 23, Issue 6, pp 419–430 | Cite as

Oviposition Marking Behavior of Diachasma alloeum, (Hymenoptera: Braconidae), Parasitizing Rhagoletis pomonella, (Diptera: Tephritidae)



Diachasma alloeum (Muesebeck) (Hymenoptera: Braconidae) is a solitary larval endoparasitoid attacking Rhagoletis (Diptera: Tephritidae) species. Rhagoletis pomonella (Walsh) mark the surface of fruit after oviposition with an oviposition marking pheromone (OMP) which deters conspecific female flies. Herein we demonstrate that female D. alloeum wasps reared from either apple or hawthorn race R. pomenella larvae also deposit an OMP that reduces oviposition by conspecific female wasps. Significantly fewer wasps accepted fruit that had received prior wasp oviposition and OMP or OMP alone without oviposition compared with control fruit for a minimum of 7 days on both fruit types. Rinsing fruit with a 50% ethanol solution appeared to remove the OMP rendering fruit more acceptable for oviposition than marked fruit that was not rinsed. Wasps of each host race were able to detect and avoid the OMP of the sister race and fruit substrate type did not affect wasp response to the pheromone. The possibility of an internal marker deposited during oviposition is also discussed.


Rhagoletis pomonella Diachasma alloeum oviposition behavior oviposition marking pheromone apple hawthorn 



A previous version of the manuscript was improved by insightful comments from two anonymous reviewers.


  1. Bush GL (1966) The taxonomy, cytology, and evolution of the genus Rhagoletis in North America (Diptera: Tephritidae). Bull Mus Comp Zool 134:431–562Google Scholar
  2. Chow A, Mackauer M (1999) Marking the package or its contents: host discrimination and acceptance in the ectoparasitoid Dendrocerus carpenteri (Hymenoptera: Megaspilidae). Can Entomol 131:495–505CrossRefGoogle Scholar
  3. Corbet SA (1971) Mandibular gland secretion of larvae of flour moth, Anagasta kuehniella, contains an epideictic pheromone and elicits oviposition movements in a hymenopteran parasite. Nature 232:481–484CrossRefPubMedGoogle Scholar
  4. Dambroski HR, Linn C, Berlocher S, Forbes AA, Roelofs WL, Feder JL (2005) The genetic basis for fruit odor discrimination in Rhagoletis flies and its significance for sympatric host shifts. Evolution 59:1953–1964PubMedGoogle Scholar
  5. Feder JL, Opp S, Wlazlo B, Reynolds K, Go W, Spisak S (1994) Host fidelity is an effective pre-mating barrier between sympatric races of the apple maggot fly. Proc Natl Acad Sci USA 91:7990–7994CrossRefPubMedGoogle Scholar
  6. Forbes AA, Powell THQ, Stelinski LL, Smith JJ, Feder JL (2009) Sequential sympatric speciation across trophic levels. Science 323:776–779CrossRefPubMedGoogle Scholar
  7. Glas PCG, Vet LEM (1983) Host-habitat location and host location by Diachasma alloeum Muesebeck (Hym.: Braconidae), a parasitoid of Rhagoletis pomonella Walsh (Dip.: Tephritidae). Neth J Zool 33:41–54CrossRefGoogle Scholar
  8. Hoffmeister TS (2000) Marking decisions and host discrimination in a parasitoid attacking concealed hosts. Can J Zool 78:1494–1499CrossRefGoogle Scholar
  9. Hoffmeister TS, Roitberg BD (1997) To mark the host or the patch: decisions of a parasitoid searching for a concealed host larvae. Evol Ecol 11:145–168CrossRefGoogle Scholar
  10. Hoffmeister TS, Roitberg BD (2002) Evolutionary ecology of oviposition marking pheromones. In: Itiker M, Meiner T (eds) Chemical ecology of insects eggs and egg deposition. Blackwell Publishing, Berlin, pp 319–347Google Scholar
  11. Holler C, Bargen H, Vinson SB, Witt D (1994) Evidence for the external use of juvenile hormone for host marking and regulation in a parasitic wasp, Dendrocerus carpenteri. J Insect Physiol 40:317–322CrossRefGoogle Scholar
  12. Landolt PJ, Averill AL (1999) Fruit flies. In: Hardie J, Minks KA (eds) Pheromones of non-lepidopteran insects associated with agricultural plants. CABI Publishing, New York, pp 3–26Google Scholar
  13. Liburd OE, Alm SR, Casagrande RA (1998) Susceptibility of highbush blueberry cultivars to larval infestation by Rhagoletis mendax (Diptera: Tephritidae) flies. Environ Entomol 27:817–821Google Scholar
  14. Linn CE, Dambroski HR, Feder JL, Berlocher SH, Nojima S, Roelofs WL (2004) Postzygotic isolating factor in sympatric speciation in Rhagoletis flies: reduced response of hybrids to parental host-fruit odors. Proc Natl Acad Sci USA 101:17753–17758CrossRefPubMedGoogle Scholar
  15. Linn CE, Nojima S, Roelofs WL (2005) Antagonistic effect of non-host fruit volatiles on discrimination of host fruit by Rhagoletis flies infesting apple (Malus pumila), hawthorn (Crataegus spp.), and flowering dogwood (Cornus florida). Entomol Exp Appl 114:97–105CrossRefGoogle Scholar
  16. Nufio CR, Papaj DR (2001) Host marking pheromone deterring repeated oviposition in apple maggot flies. Entomol Exp Appl 99:273–293CrossRefGoogle Scholar
  17. Prokopy RJ (1981a) Epidiectic pheromones that influence spacing patterns of phytophagous insects. In: Nordlund DA, Jones RL, Lewis WJ (eds) Semiochemicals: their role in pest control. Wiley, New York, pp 181–213Google Scholar
  18. Prokopy RJ (1981b) Oviposition-deterring pheromone system of apple maggot flies. In: Mitchell EK (ed) Management of insect pests with semiochemicals. Plenum Press, New York, pp 477–494Google Scholar
  19. Roitberg BD, Prokopy RJ (1987) Insects that mark host plants. Bioscience 37:400–406CrossRefGoogle Scholar
  20. Stelinski LL, Liburd OE (2005) Behavioral evidence for host fidelity among populations of the parasitic wasp, Diachasma alloeum (Muesebeck). Naturwissenschaften 92:65–68CrossRefPubMedGoogle Scholar
  21. Stelinski LL, Pelz KS, Liburd OE (2004) Field observations quantifying attraction of the parasitic wasp, Diachasma alloeum (Hypmenoptera: Braconidae) to blueberry fruit infested by the blueberry maggot fly, Rhagoletis alloeum (Diptera: Tephritidae). Fla Entomol 87:124–129CrossRefGoogle Scholar
  22. Stelinski LL, Pelz-Stelinski KS, Liburd OE, Gut LJ (2006) Control strategies for Rhagoletis mendax disrupt host-finding and oviposition capability of its parasitic wasp, Diachasma alloeum. Biol Control 36:91–99CrossRefGoogle Scholar
  23. Stelinski LL, Oakleaf R, Rodriguez-Saona C (2007) Oviposition-deterring pheromone deposited on blueberry fruit by the parasitic wasp, Diachasma alloeum. Behaviour 144:429–445CrossRefGoogle Scholar
  24. van Lenteren JC (1981) Host discrimination by parasitoids. In: Nordlund DA, Jones RL, Lewis WJ (eds) Semiochemicals: their role in pest control. Wiley, New York, pp 153–173Google Scholar
  25. Wharton RA, Marsh PM (1978) New world Opiinae (Hymenoptera: Braconidae) parasitic on Tephritidae (Diptera). J Wash Acad Sci 68:147–167Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Lukasz L. Stelinski
    • 1
  • D. R. Boina
    • 1
  • W. L. Meyer
    • 1
  1. 1.Entomology and Nematology Department, Citrus Research and Education CenterUniversity of FloridaLake AlfredUSA

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