Journal of Insect Behavior

, Volume 21, Issue 4, pp 222–239 | Cite as

Behavioral Sequence Leading to Sexual Isolation Between Drosophila ananassae and D. pallidosa

  • Hirokazu Yamada
  • Masatoshi Tomaru
  • Muneo Matsuda
  • Yuzuru Oguma


Drosophila ananassae and D. pallidosa are closely related, sympatric species that lack postmating isolation. Sexual isolation has been considered important in maintaining them as independent species. To clarify the behavioral processes leading to sexual isolation, we analyzed behavioral sequences and examined the effect of courtship song on mating success and on behaviors of both sexes by surgically removing male wings (song generators), female aristae (song receivers), or female wings (means of fluttering). We found that heterospecific courtship songs evoked female wing fluttering, whereas conspecific courtship song did not. Furthermore, female wing fluttering made courting males discontinue courtship. These findings suggest that strong sexual isolation is achieved through the following behavioral sequence: heterospecific song→female wing fluttering→courtship discontinuation.


Drosophila ananassae Drosophila pallidosa sexual isolation speciation courtship song female rejection 



We thank Dr Y. N. Tobari for providing strains and helpful comments and Dr M. G. Ritchie and anonymous reviewers for kindly providing valuable comments on the manuscript. This research was supported by Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to YO. MM also thanks National Bio-Resource Project (NBRP) of MEXT for supporting him for Drosophila resources.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Hirokazu Yamada
    • 1
    • 4
  • Masatoshi Tomaru
    • 2
  • Muneo Matsuda
    • 3
  • Yuzuru Oguma
    • 1
  1. 1.Institute of Biological SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Drosophila Genetic Resource Center, Kyoto Institute of TechnologyUkyo-kuJapan
  3. 3.Department of Biology, School of MedicineKyorin UniversityMitakaJapan
  4. 4.Program for Genetic Resource Education and Development, Drosophila Genetic Resource Center, Kyoto Institute of TechnologyUkyo-kuJapan

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