Spectroscopic and Electrochemical Features of Glucose Oxidase Incorporation into Polyaniline-Cobaltous Oxalate Nano-complex
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Nano-complex as enzyme carrier was prepared via hybridization of nano-scale cobaltous oxalate with conductive polymer: polyaniline. Spectrometric means and electrochemical technique were proposed to explore the influence of interaction between nano-composite and redox protein on structural features, dynamics of electron shuttle and catalysis of immobilized enzyme. Results indicated that collaboration of π–π stacking effect as well as the complexation of metal ions in nano-composite with cofactors in enzyme molecule could lead to the firm linkage of glucose oxidase to the matrix of nano-composite. The ligation would cause orientated attachment of enzyme molecules onto the surface of nano-complex. Synergistic effect would not only enhance the enzyme loading capacity of carrier but also facilitate the direct electrochemistry of tethered enzyme molecules with intact structure and configuration of cofactor. However the complexation would cripple the enzymatic catalysis in electro-oxidation of glucose in spite of high affinity to glucose for glucose oxidase integration into nano-composite.
Coordination of metal ions within nano-composite with cofactor of glucose oxidase (GOx) would lead to formation of composite with crippled emission fluorescence and hinder the electro-oxidation of glucose.
KeywordsNano-complex Polyaniline Nano-scale CoC2O4 Coordination Glucose oxidase Spectroscopic feature Enzymatic oxidation of glucose
The study was financially supported by the National Natural Science Foundation of China (Nos. 31560249, 21363024).
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