A Novel Ship-in-Bottle Type Immobilized HRP via Co-adsorption of Super Paramagnets and HRP into Silica Hollow Fiber
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In this paper, two type silica hollow fibers were synthesized through a soft template and hard template directing route respectively, and the super paramagnets was synthesized by thermal decomposition. The obtained silica hollow fiber and super paramagnets were characterized by XRD, N2 adsorption, SEM, TEM, EDS and XPS respectively. The ship-in-bottle type immobilized horseradish peroxidase (HRP) was assembled by co-adsorption of super paramagnets and HRP into the pore channel of silica hollow fiber in reverse microemulsion media. The effects of various parameters on enzyme activity of ship-in-bottle type immobilized HRP, including morphology, water content (ω0), super paramagnets concentration, HRP concentration, adsorption time, pH, crosslinking time and glutaraldehyde concentration were discussed in detailed. Moreover, the degradation performance of dichlorodiphenyltrichloroethane (DDT) by HRP immobilized on silica hollow fiber and spherical nano-silica was evaluated.
KeywordsSilica hollow fiber Super paramagnets Immobilized horseradish peroxidase Ship-in-bottle
This work was supported by the National Natural Science Foundation of China (20971043), State Key Laboratory of Catalytic Materials and Reaction Engineering (RIPP, SINOPEC), the Open Project Program of State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, and the Fundamental Research Funds for the Central Universities.
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