Anti-neoplastic Applications of Heparin Coated Magnetic Nanoparticles Against Human Ovarian Cancer

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Abstract

This study determines the effect of bare and heparin (HP)-based magnetic iron oxide (Fe3O4) nanoparticles (MNPs) on the human ovarian cancer cells of CP70 type. Cisplatin (CP) was used as the anticancer drug, entrapped in MNPs. The nanoparticles containing the anticancer drug, CP, were prepared by a solvent evaporation and emulsification cross-linking method. The physicochemical properties of the nanoparticles were characterized by various techniques, and uniform particles of bare and HP coated MNPs, with average particle sizes of 25 and 45 ± 15 nm, having high encapsulation efficiencies, were obtained. Additionally, a sustained release of CP from the MNPs was successful in vitro. Cytotoxicity tests showed that the MNP–HP–CP had higher cell toxicity than the individual HP; and confocal microscopic analysis confirmed excellent cellular uptake efficiency of CP70 cells for MNP–HP–CP. These results indicate that HP based MNPs have potential uses as anticancer drug carriers and have also proved to have enhanced anti-neoplastic effects.

Keywords

Heparin CP70 cells Ovarian cancer Nanoparticles Cisplatin 
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of BiochemistryPayame Noor UniversityEsfahānIran

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