Bioimaging Applications of Carbon dots (C. dots) and its Cystamine Functionalization for the Sensitive Detection of Cr(VI) in Aqueous Samples

  • Roshni V.
  • Varsha Gujar
  • Heena Pathan
  • Sehbanul Islam
  • Madhumita Tawre
  • Karishma Pardesi
  • Manas Kumar Santra
  • Divya OttoorEmail author


In this study, one step hydrothermal synthetic strategy was adopted for preparing carbon dots (C. dots) from jeera (Cumin: Cuminum cyminum), a naturally abundant and cost effective carbon source. The physical, optical and surface functional properties of C. dots were extensively studied by different techniques such as Transmission electron microscopy (TEM), Scanning electron microscopy (SEM), spectrophotometry, fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The obtained C. dots were highly water dispersible and photostable with a quantum yield of 5.33%. The antioxidant property of C. dots was investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The C. dots were then capped with cystamine using 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) coupling chemistry to design a selective sensing system for chromium (VI) (Cr (VI)). The minimum detection limit of Cr (VI) was found to be 1.57 μM. Biocompatibility and low toxicity of C. dots obtained from jeera made it a potential tool for bioimaging application. The internalisation of C. dots by MCF-7 breast cancer cells and Multi Drug Resistant (MDR) pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa were proved by the bioimaging of respective cells.


Carbon dots (C. dots) Cumin seeds (jeera) Fluorescence Cr (VI) sensing MCF-7 cells Bioimaging 



Physics department, Savitribai Phule Pune University, Pune is acknowledged for TEM facility.

Supplementary material

10895_2019_2448_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1.2 mb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Roshni V.
    • 1
  • Varsha Gujar
    • 1
  • Heena Pathan
    • 1
  • Sehbanul Islam
    • 2
  • Madhumita Tawre
    • 3
  • Karishma Pardesi
    • 3
  • Manas Kumar Santra
    • 2
  • Divya Ottoor
    • 1
    Email author
  1. 1.Department of ChemistrySavitribai Phule Pune UniversityPuneIndia
  2. 2.National Centre For Cell Science(NCCS)PuneIndia
  3. 3.Department of MicrobiologySavitribai Phule Pune UniversityPuneIndia

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