Advertisement

Determination of Ondansetron by Spectrofluorimetry: Application to Forced Degradation Study, Pharmaceuticals and Human Plasma

  • Sambhani Naga Gayatri
  • Valsala Madhavan Nair Biju
  • Ambrose Maria Starvin
ORIGINAL ARTICLE
  • 32 Downloads

Abstract

The current manuscript describes a validated, responsive and rapid spectrofluorimetric method for quantifying ondansetron (OND) in authentic form, spiked human plasma and dosage forms. This is the first reported fluorescence study of Ondansetron in Triton X 100 system. Various variables affecting fluorescence response were studied precisely and optimised. The described method involved the fluorescence measurement in Triton X 100 system at λemex 354/317 nm. The calibration plot attained linearity over concentration range of 0.2 – 2 μg/mL. The developed method has been extensively applied to degradation studies of OND as per International Conference on Harmonisation (ICH) guidelines by exposing to oxidative, thermal, photo, acidic and alkaline conditions and also the degradation pathway has been proposed.

Keywords

Ondansetron Spectrofluorimetry Pharmaceutical dosage forms Spiked human plasma Stability studies 

References

  1. 1.
    Sweetman SC (2009) Martindale: the complete drug reference. Pharmaceutical Press, London.  https://doi.org/10.3163/1536-5050.100.1.018 CrossRefGoogle Scholar
  2. 2.
    Currow DC, Coughlan M, Fardell B, Cooney NJ (1997) J Pain Symptom Manage 13:302–307.  https://doi.org/10.1016/S0885-3924(97)00079-1 CrossRefPubMedGoogle Scholar
  3. 3.
    Indian Pharmacopoeia (2010) Published by the controller & publication, Delhi. 1819: 2045Google Scholar
  4. 4.
    British Pharmacopoeia (2007) Her Majesty’s stationary office, London, p.1524–1525Google Scholar
  5. 5.
    The United State Pharmacopoeia (2000) 24 th ed. Philadelphia: National Publishing; p. 1218Google Scholar
  6. 6.
    Rao K, Jayachandran E, Usha M, Gopinath G, Venkata RD, Srinivasa Rao D (2012) Int J Pharm Pharm Sci 4:125–129Google Scholar
  7. 7.
    Kumar PR, Krishna MM, Prakash PB, Kumar BA, Madhusudhan P (2006) E-Journal of Chemistry 3:134–136.  https://doi.org/10.1155/2006/245321 CrossRefGoogle Scholar
  8. 8.
    Patra S, Choudary AA, Kar RK, Barik BB (2007) Indian J Pharm Sci 69:840–841.  https://doi.org/10.4103/0250-474X.39449 CrossRefGoogle Scholar
  9. 9.
    Mujtaba A, Kohli K, Ali J, Baboota S (2013) Drug Test Anal 5:122–125.  https://doi.org/10.1002/dta.311 CrossRefPubMedGoogle Scholar
  10. 10.
    Raval PB, Puranik M, Wadher SJ, Yeole PG (2008) A validated HPTLC method for determination of ondansetron in combination with omeprazole or rabeprazole in solid dosage form. Indian J Pharm Sci 70:386–390.  https://doi.org/10.4103/0250-474X.43011 CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Dedania Z, Dedania R, Karkhanis V, Sagar GV, Baldania M, Sheth NR (2009) AJRC 2:108–111Google Scholar
  12. 12.
    Meyyanathan SN, Venkatesh DN, Krishnaveni N, Babu B, Jeyaprakash MR, Raja RB, Hemnath E, Suresh B (2012) A RP-HPLC method for simultaneous estimation of ondansetron and ranitidine in pharmaceutical formulation. Int J Health Allied Sci 1:129–132.  https://doi.org/10.4103/2278-344X.101723 CrossRefGoogle Scholar
  13. 13.
    Patel SR, Patel LJ, Thakkar YP, Patel ND (2010) Int J Chemtech Res 2:1531–1536Google Scholar
  14. 14.
    Bauer S, Stormer E, Kaiser R, Tremblay PB, Brock-moller J, Roots I (2002) Biomed Chromatogr 16:187–190.  https://doi.org/10.1365/s10337-009-1117-0009-5893/09/07 CrossRefPubMedGoogle Scholar
  15. 15.
    Chandrasekar D, Ramakrishna S, Diwan PV (2004) Arzneim Forsch 54:655–659.  https://doi.org/10.1055/s-0031-1297017 CrossRefGoogle Scholar
  16. 16.
    Zheng HS, Pan W, Wang Y, Mao SR, Bi DZ (2002) Chinese Journal of Pharmaceuticals 33:603–604Google Scholar
  17. 17.
    Abdel-Hamid ME, Sharma D (2005) J Liq Chromatogr Relat Technol 27:641–660.  https://doi.org/10.1081/JLC-120028254 CrossRefGoogle Scholar
  18. 18.
    Pang L, Wang Q, Wang Y, Zheng M, Wu H, Wang Z (2012) Lat Am J Pharm 31:305–309Google Scholar
  19. 19.
    Ravi S, Khan N, Darwis Y (2011) Development and validation of an RP-HPLC-UV method for the determination of ondansetron in rabbit plasma: Application to a pharmacokinetic study. Acta Chromatogr 23:579–593.  https://doi.org/10.1556/AChrom.23.2011.4.4 CrossRefGoogle Scholar
  20. 20.
    Tang B, Wang X, Jia B, Niu J, Ying Wei Y, Chen Z, Wang Y (2003) Simple, Rapid, and Sensitive Spectrofluorimetric Determination of Zaleplon in Micellar Medium. Anal Lett 36:2985–2997CrossRefGoogle Scholar
  21. 21.
    Belal F, Ibrahim F, Sheribah Z, Alaa H (2017) Luminescence 1-8.  https://doi.org/10.1002/bio.3325 CrossRefGoogle Scholar
  22. 22.
    Ibrahim F, El-Enany N, Shalan S, Elsharawy R (2016) Luminescence 31:682.  https://doi.org/10.1002/bio.3011 CrossRefPubMedGoogle Scholar
  23. 23.
    Mandal B, Balabathula P, Mittal N, Wood GC (2012) Development and Validation of a Spectrofluorimetric Method for the Determination of Erlotinib in Spiked Human Plasma. J Fluoresc 22:1425–1429.  https://doi.org/10.1007/s10895-012-1103-8 CrossRefPubMedGoogle Scholar
  24. 24.
    Mohamed A-MI, Omar MA, Hammad MA, Mohamed AA (2016) Development and Validation of highly Sensitive Stability Indicating Spectrofluorimetric Method for Determination of Amlodipine in Pharmaceutical Preparations and Human Plasma. J Fluoresc 26:2141–2149.  https://doi.org/10.1007/s10895-016-1910-4 CrossRefPubMedGoogle Scholar
  25. 25.
    International Conference on Harmonisation guideline, Q1A (R2) Stability Testing of New Drug Substances and Products, IFPMA, Geneva, Switzerland 2003Google Scholar
  26. 26.
    International Conference on Harmonisation guideline, Q2 (R1): Validation of Analytical Procedures: Text and Methodology, IFPMA, Geneva, Switzerland 2005Google Scholar
  27. 27.
    Hinze WL, Singh HN, Baba Y, Harvey NG (1984) Micellar enhanced analytical fluorimetry. TrAC Trends Anal Chem 3:193–199CrossRefGoogle Scholar
  28. 28.
    McIntire GL (2007) Micelles in analytical chemistry. Crit Rev Anal Chem 21:257–278.  https://doi.org/10.1080/10408349008051631 CrossRefGoogle Scholar
  29. 29.
    Wang CC, Masi AN, Fernandez L (2008) Talanta 75:135–140.  https://doi.org/10.1016/j.talanta.2007.10.041 CrossRefPubMedGoogle Scholar
  30. 30.
    Skoog DA, Holler FJ, Crouch SR (2007) Principles of instrumental analysis, 6th edn. Thomson, Belmont, p 406Google Scholar
  31. 31.
    Leung R, Shah DO (1986) Dynamic properties of micellar solutions. Colloid Interface Sci 113:484–499CrossRefGoogle Scholar
  32. 32.
    Skoog DA, West DM, Holler FJ, Crouch SR (2004) Editor#. Fundamentals of analytical chemistry, 8th edn. Saunders College Publishing, Philadelphia, pp 1003–1006Google Scholar
  33. 33.
    Miller JN, Miller JC (2005) Statistics and chemo metrics for analytical chemistry, vol 39–73, 5th edn. Pearson Education, Harlow, pp 107–149 256Google Scholar
  34. 34.
    Kumar Talluri MVN, Keshari KK, Kalariya PD, Srinivas R (2015) J Sep Sci 38:1625–1632.  https://doi.org/10.1002/jssc.201401305 CrossRefGoogle Scholar
  35. 35.
    Patel DR, Joshi A, Patel HH, Stagni G (2015) Development andin-vivoevaluation of ondansetron gels for transdermal delivery. Drug Dev Ind Pharm 41:1030–1036.  https://doi.org/10.3109/03639045.2014.925916 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sambhani Naga Gayatri
    • 1
  • Valsala Madhavan Nair Biju
    • 1
  • Ambrose Maria Starvin
    • 2
  1. 1.Department of ChemistryNational Institute of TechnologyTrichyIndia
  2. 2.Department of ChemistryChristian CollegeThiruvananthapuramIndia

Personalised recommendations