Journal of Fluorescence

, Volume 28, Issue 6, pp 1305–1315 | Cite as

Synthesis and Studies of Fluorescein Based Derivatives for their Optical Properties, Urease Inhibition and Molecular Docking

  • Prasad G. Mahajan
  • Nilam C. Dige
  • Balasaheb D. Vanjare
  • Hussain Raza
  • Mubashir Hassan
  • Sung-Yum Seo
  • Seong-Karp Hong
  • Ki Hwan LeeEmail author


Herein, we design and synthesized new fluorescein based derivatives by insitu formation of fluorescein ester and further treated with corresponding hydrazide and amine to yield respective compounds i.e. FB1, FB2, FB3 and FB4. The spectral purity and characterization was done by using IR, NMR and Mass spectroscopies. The synthesized derivatives were examined for their photophysical properties by using variety of organic solvents and results were discussed in details. The structural diversity of synthesized compounds motivate us to evaluate these compounds for urease inhibition. The compound FB3 (IC50 = 0.0456 μM) shows 100 fold more active against Jack bean urease than standard drug thiourea (IC50 = 4.7455 μM). Other synthesized compounds showed potent activity. Free radical percentage scavenging assay further supported the capacity of compounds to urease inhibition. While, molecular docking simulations helps to examine the molecular interactions of active compounds FB1, FB2, FB3 and FB4 within the binding site of urease enzyme.


Synthesis Fluorescein derivatives Optical properties Urease inhibitors Molecular docking 



This work was supported by a grant from Mid-Career Researcher Program (NRF- 2016R1A2B4016552) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (MSIP).

Supplementary material

10895_2018_2291_MOESM1_ESM.docx (1.4 mb)
ESM 1 (DOCX 1.37 mb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018
corrected publication September/2018

Authors and Affiliations

  • Prasad G. Mahajan
    • 1
  • Nilam C. Dige
    • 2
  • Balasaheb D. Vanjare
    • 1
  • Hussain Raza
    • 3
  • Mubashir Hassan
    • 3
  • Sung-Yum Seo
    • 3
  • Seong-Karp Hong
    • 4
  • Ki Hwan Lee
    • 1
    Email author
  1. 1.Department of ChemistryKongju National UniversityChungnamRepublic of Korea
  2. 2.Department of ChemistryShivaji UniversityKolhapurIndia
  3. 3.Department of Biological SciencesKongju National UniversityChungnamRepublic of Korea
  4. 4.Division of Bio and Health SciencesMokwon UniversityDaejeonRepublic of Korea

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