A Novel Fluorescent Nanoparticle for Sensitive Detection of Cry1Ab Protein In Vitro and In Vivo
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Here, we report the synthesis and characterization of CoFe2O4 doping Ag2S dendrimer-modified nanoparticles (CoFe2O4-Ag2S DMNs) in Cry1Ab protein detection and imaging. The near-infrared Ag2S quantum dots were first prepared by using the thermal decomposition method, followed by modification of the water-soluble quantum dots using the method of solvent evaporation and ligand exchange, and finally the fluorescent magnetic bifunctional nanoparticles were obtained by binding with CoFe2O4. As-prepared CoFe2O4-Ag2S DMNs were characterized by fluorescence (FL) spectroscopy and transmission electron microscopy (TEM). Results showed that Ag2S DMNs could sensitively detect Cry1Ab both in vitro and in vivo. In vitro, the enhanced FL intensity as a function of the concentration is notably consistent with the Langmuir binding isotherm equation in the range of 0–200 ng/mL of Cry1Ab proteins. The detection limit of this method was found to be 0.2 ng/mL. Meanwhile, the fluorescence wavelength was extended to the second near-infrared range (NIR-II, 1.0~1.4 μm), which enables in vivo imaging. This study highlights the importance of NIR QDs doping magnetic materials as a new method to trace Bacillus thuringiensis (Bt) in insects and their potential applications in in vivo NIR tissue imaging.
KeywordsAg2S quantum dot Bacillus thuringiensis PAMAM CoFe2O4 Cry1Ab protein
This project was supported by the Scientific Research Initial funding for the advanced talent of Jianghan University (08010001, 06750001); Dr. Liu thanks the supported from the Fundamental Research Funds for the Central Universities (2010QC014) and Natural Science Foundation of Hubei Province of China (2014CFA092) and Fundamental Research Funds for the Central Universities (Program No.2662018PY018).
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