Journal of Fluorescence

, Volume 26, Issue 4, pp 1407–1414 | Cite as

Ligand Assisted Stabilization of Fluorescence Nanoparticles; an Insight on the Fluorescence Characteristics, Dispersion Stability and DNA Loading Efficiency of Nanoparticles

  • Amina Rhouati
  • Akhtar Hayat
  • Rupesh K. Mishra
  • Diana Bueno
  • Shakir Ahmad Shahid
  • Roberto Muñoz
  • Jean Louis Marty
ORIGINAL ARTICLE
  • 151 Downloads

Abstract

This work reports on the ligand assisted stabilization of Fluospheres® carboxylate modified nanoparticles (FCMNPs), and subsequently investigation on the DNA loading capacity and fluorescence response of the modified particles. The designed fluorescence bioconjugate was characterized with enhanced fluorescence characteristics, good stability and large surface area with high DNA loading efficiency. For comparison purpose, bovine serum albumin (BSA) and polyethylene glycol (PEG) with three different length strands were used as cross linkers to modify the particles, and their DNA loading capacity and fluorescence characteristics were investigated. By comparing the performance of the particles, we found that the most improved fluorescence characteristics, enhanced DNA loading and high dispersion stability were obtained, when employing PEG of long spacer arm length. The designed fluorescence bioconjugate was observed to maintain all its characteristics under varying pH over an extended period of time. These types of bioconjugates are in great demand for fluorescence imaging and in vivo fluorescence biomedical application, especially when most of the as synthesized fluorescence particles cannot withstand to varying in vivo physiological conditions with decreases in fluorescence response and DNA loading efficiency.

Keywords

Fluorescence particles Fluorescence bioconjugates Dispersion and stability DNA loading efficiency Polyethylene glycol crosslinkers 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Amina Rhouati
    • 1
    • 2
  • Akhtar Hayat
    • 1
    • 3
  • Rupesh K. Mishra
    • 1
  • Diana Bueno
    • 1
    • 4
  • Shakir Ahmad Shahid
    • 5
  • Roberto Muñoz
    • 4
  • Jean Louis Marty
    • 1
  1. 1.BAE: Biocapteurs-Analyses-EnvironnementUniversite de Perpignan Via DomitiaPerpignanFrance
  2. 2.Ecole Nationale Supérieure de BiotechnologieConstantineAlgérie
  3. 3.Interdisciplinary Research centre in Biomedical Materials (IRCBM)COMSATS Institute of Information TechnologyLahorePakistan
  4. 4.Bioelectronics Section, Department of Electrical EngineeringCINVESTAV-IPNMexicoMexico
  5. 5.Department of ChemistryUniversity of SargodhaSargodhaPakistan

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