A Turn on ESIPT Probe for Rapid and Ratiometric Fluorogenic Detection of Hg2+ and its Application in Live-Cell Imaging
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A probe based on 2-(2′-hydroxyphenyl) benzothiazole (HBT) and thiophosphate has been synthesized and used for the ratiometric detection of Hg2+. The probe was designed in such a way that the excited state intramolecular proton transfer (ESIPT) of the HBT moiety get blocked. The probe exhibited a strong fluorescence enhancement upon addition of Hg2+ while showing almost no response to other cations in CH3CN/HEPES buffer solution. The probe exhibited fast selectivity towards Hg2+ and could be completed in 1 min. Fluorescence imaging experiments of Hg2+ in living TE-1 cells demonstrated its value of practical applications in biological systems.
KeywordsHg2+ ESIPT Thiophosphate Fluorescence Deprotection
This work was supported by the National Science Foundation of China (Nos. 21305031), Scientific and Technological Project of Henan Province of China (152102110135 and 162102310352), Science-Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences (Grant no. 2016YQ22).
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