2-Carbamido-1,3-indandione – a Fluorescent Molecular Probe and Sunscreen Candidate
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The present work reports theoretical and experimental studies on the photophysical properties of two tautomeric forms of 2-carbamido-1,3-indandione (CAID). By means of UV-vis, steady-state and time-dependent fluorescence spectroscopy it is shown that both enol forms, 2-(hydroxylaminomethylidene)-indan-1,3-dione and 2-carboamide-1-hydroxy-3-oxo-indan, coexist in solution. On the base of spectroscopic studies of CAID interaction with human serum albumin and DNA sequences, it was shown that the compound has potential and it is suitable for use as fluorescent molecular probe for investigation of different biomolecules. CAID shows relatively high photostability within 3 h irradiation period. Such behavior of the investigated compound supposes possibilities for using of the CAID molecule as sunscreen because of strong absorption in UVA, UVB and UVC light spectra.
Keywords2-carbamido-1,3-indandion Tautomerism Fluorescence Sunscreen
The authors are grateful to Dr. Ekaterina Borisova and Ms. Alexandra Zhelyazkova, Institute of Electronics, Bulgarian Academy of Sciences for technical support. Two of the authors (IA and VM) acknowledge the financial support of the National Science Fund under grant DFNI-02/9/2014.
- 5.Lakowicz JR (2006) Principles of Fluorescence Spectroscopy. Springer, Third EditionGoogle Scholar
- 9.Zhao J, Ji S, Chen Y, Guo H, Yang P (2012) Excited state intramolecular proton transfer (ESIPT): from principal photophysics to the development of new chromophores and applications in fluorescent molecular probes and luminescent materials. Phys Chem Chem Phys 14:8803–8817CrossRefPubMedGoogle Scholar
- 33.Bohren CF, Huffman DR (1983) Absorption and scattering of light by small particles. John Wiley and Son Inc., New YorkGoogle Scholar