Analysis of Quantum Rod Diffusion by Polarized Fluorescence Correlation Spectroscopy
To measure the polarization dependence of fluorescent probes, a confocal-microscope-based polarized fluorescence correlation spectroscopy system was developed, and the polarization dependence on the rotational diffusion of well-defined quantum rods (Qrods) was investigated and characterized. The rotational diffusion region of the Qrods was observed over a time range of less than 10−5 s in a water solution, and the rotational diffusion parameters were extracted using a rotational diffusion model in which the viscosity of the solution media was varied. Our work demonstrated that polarized fluorescence correlation spectroscopy (FCS) is useful for investigating both the rotational and translational diffusion of fluorescent probes.
KeywordsPolarization Spectroscopy Fluorescence Luminescence Correlators
This work was supported by a grant from the National Research Foundation of Korea, funded by the Korean Government (No. NRF 2012R1A1A20044140) and by the Priority Research Centers Program through the National Pesearch Foundation of Korea (NRF) funded by the Ministry of Education (2009-0093818).
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