Journal of Fluorescence

, Volume 23, Issue 3, pp 551–559

A Straightforward Immunoassay Applicable to a Wide Range of Antibodies Based on Surface Enhanced Fluorescence

  • Ruohu Zhang
  • Zhuyuan Wang
  • Chunyuan Song
  • Jing Yang
  • Yiping Cui


A straightforward immunoassay based on surface enhanced fluorescence (SEF) has been demonstrated using a fluorescent immune substrate and antibody functionalized-silver nanoparticles. Unlike the conventional SEF-based immunoassay, which usually uses the dye-labeled antibodies and the metallic nanostructured-substrates, the presented immune system does not need the antibodies to be labeled with dye molecules. Thus, this immunoassay can be easily applied to the detection of a wide range of target antigens, which is of great importance for its practical application. The experimental results show that this immunoassay has a good specificity as well as the capacity of quantitative detection. Basically, the surface density of the immuno-adsorbed silver nanoparticles increases with the increased amount of target antigens, resulting in a fluorescence enhancement up to around 7 fold. The dose-responsive performance of the immunoassay has been investigated and the limit of detection (LOD) is 1 ng/mL. Due to its simple preparation method and the wide range of detectable antigens, this presented immunoassay is expected to be helpful for extending the SEF-based application.


Surface enhanced fluorescence Surface plasmon resonance Immunoassay Ag nanoparticle Substrate 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ruohu Zhang
    • 1
  • Zhuyuan Wang
    • 1
  • Chunyuan Song
    • 1
  • Jing Yang
    • 1
  • Yiping Cui
    • 1
  1. 1.Advanced Photonics CenterSoutheast UniversityNanjingPeople’s Republic of China

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