Journal of Fluorescence

, Volume 22, Issue 6, pp 1571–1579

The Application of CdTe/CdS in the Detection of Carcinoembryonic Antigen by Fluorescence Polarization Immunoassay

  • Jianniao Tian
  • Liujin Zhou
  • Yanchun Zhao
  • Yuan Wang
  • Yan Peng
  • Xue Hong
  • Shulin Zhao
ORIGINAL PAPER

Abstract

A novel and portable strategy based on fluorescence polarization immunoassay (FPIA) using quantum dots (QDs) was described in this study for simple, rapid, and sensitive detection of carcinoembryonic antigen (CEA). Under optimal conditions, the sensor has a wide dynamic range (from 0.5 ng/mL to 200 ng/mL) and a good correlation. The limit of detection (LOD) is 0.21 ng/mL (S/N = 3). The sensor has been applied for detection of carcinoembryonic antigen in 10 human serum samples with the range of recovery from 92.1 % to 103.6 %. Furthermore, bioconjugation of the core–shell QDs with streptavidin (SA) has been successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HEPG2) cell line. The experimental results demonstrated the successful application of QDs-based fluorescence polarization immunoassay for detection of target proteins of biomedical importance. This strategy shows great promise for clinical diagnoses and basic discovery with high sensitivity, good specificity, simple procedures and short analysis time.

Keywords

Fuorescence polarization immunoassay Core–shell quantum dots Carcinoembryonic antigen Cell imaging 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jianniao Tian
    • 1
  • Liujin Zhou
    • 1
  • Yanchun Zhao
    • 1
  • Yuan Wang
    • 1
  • Yan Peng
    • 1
    • 2
  • Xue Hong
    • 1
    • 2
  • Shulin Zhao
    • 1
  1. 1.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China)College of Chemistry and Chemical Engineering of Guangxi Normal UniversityGuilinChina
  2. 2.College of Life ScienceGuangxi Normal UniversityGuilinChina

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