Journal of Fluorescence

, Volume 22, Issue 4, pp 1055–1063 | Cite as

Fluorescent Function-Spacer-Lipid Construct Labelling Allows for Real-Time in Vivo Imaging of Cell Migration and Behaviour in Zebrafish (Danio Rerio)

  • Chuan-Ching Lan
  • Deborah Blake
  • Stephen Henry
  • Donald R. LoveEmail author


Real-time in vivo imaging of cell migration and behavior has advanced our understanding of physiological processes in situ, especially in the field of immunology. We carried out the transplantation of a mixed population of blood cells from adult zebrafish (Danio rerio) to 2 day old embryos. The blood cells were treated ex vivo with Function-Spacer-Lipid constructs (FSL) incorporating either fluorescein or Atto488 fluorophores (FSL-FLRO4-I or -II). Excellent labeling efficiency was demonstrated by epifluorescence microscopy and FACScan analysis. Real-time video imaging of the recipient fish showed that the functionality of these cells was retained and not affected by the labeling. The usefulness of FSL-FLRO4-I as a contrast agent in microangiography was explored. Overall, we found both FSL-FLRO4-I and-II promising labeling dyes for real-time in vivo imaging in zebrafish.


Kodecyte Labeling Fluorescence Zebrafish 



Professor Nicolai Bovin’s team from the Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, is gratefully acknowledged for preparing FSL-FLRO4-II.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Chuan-Ching Lan
    • 1
  • Deborah Blake
    • 2
  • Stephen Henry
    • 2
  • Donald R. Love
    • 1
    • 3
    Email author
  1. 1.School of Biological SciencesUniversity of AucklandAucklandNew Zealand
  2. 2.Biotechnology Research InstituteAUT UniversityAucklandNew Zealand
  3. 3.LabPLUSAuckland City HospitalAucklandNew Zealand

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