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Journal of Fluorescence

, Volume 19, Issue 3, pp 389–397 | Cite as

Quenching of Long Lifetime Emitting Fluorophores with Paramagnetic Molecules

  • O. Oter
  • A-C. RibouEmail author
Original Paper

Abstract

In this work, we have studied quenching of the fluorescence of two well-known oxygen probes, 1-pyrene butyric acid (PBA) and tris(2,2’-bipyridine)ruthenium ([Ru(bpy)3]2+) by reactive oxygen species (superoxide anion, nitric oxide derivative, hydrogen peroxide) and by the O2 molecule. Both, time-resolved and steady state fluorescence measurements were performed in solution (ethanol, dimethyl sufoxide, water) and in micelles of Sodium Dodecyl Sulfate that serve as a model for membrane-containing biological structures. We have found that only the free radicals and O2 can actively quench for the two probes, but not the diamagnetic H2O2. Our data correspond to the classical Stern–Volmer equation. H2O2 has an effect only at high molar concentrations (>0.1 M). In contrast, effective concentrations of free radicals and O2 that lead to quenching are in millimolar range. In conclusion, our methods allows for detecting global ROS that are small free radicals without interference from the reactive hydroxyl radical. Our data suggest that the method can be used for the quantification of ROS in individual living cells based on the measurement of fluorescence lifetime of those probes.

Keywords

Fluorescence quenching Oxygen probes Reactive oxygen species 

Notes

Acknowledgments

This work was financially supported by the French “Ligue Contre le Cancer- Comité départemental du Gard”.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of Dokuz EylulIzmirTurkey
  2. 2.Institut de Modélisation et d’Analyse en Géo-Environnements et SantéUniversité de Perpignan Via DomitiaPerpignanFrance

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