Journal of Fluorescence

, Volume 18, Issue 1, pp 61–74 | Cite as

Second-derivative Synchronous Fluorometric Method for the Simultaneous Determination of Cinnarizine and Domperidone in Pharmaceutical Preparations. Application to Biological Fluids

  • M. I. Walash
  • F. Belal
  • N. El-Enany
  • A. A. Abdelal
Original Paper

Abstract

A rapid, simple and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixture of cinnarizine (CN) and domperidone (DOM). The method is based upon measurement of the native fluorescence of these drugs at Δλ = 80 nm in aqueous methanol (50% V/V). The different experimental parameters affecting the native fluorescence of the studied drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.1 to 1.3 μg mL−1 and 0.1–3.0 μg mL−1 for CN and DOM, respectively with lower detection limits of 0.017 and 5.77 × 10−3 μg mL−1 and quantification limits of 0.058 and 0.02 μg mL−1 for CN and DOM. The proposed method was successfully applied for the determination of the studied compounds in synthetic mixtures and in commercial tablets. The results obtained were in good agreement with those obtained with reference methods. The high sensitivity attained by the synchronous fluorometric method allowed the determination of CN in real and spiked human plasma. The mean % recoveries in case of spiked human plasma (n = 3) were 96.39 ± 1.18 while that in real human plasma (n = 3) was 104.67 ± 4.16.

Keywords

Cinnarizine Domperidone Second derivative synchronous fluorimetry Pharmaceutical preparations and biological fluids 

References

  1. 1.
    Budavari S (1996) The Merck index, 12th edn. Merck, White House StationGoogle Scholar
  2. 2.
    Parfitt K (1999) Martindale, the complete drug reference, 32th edn. Pharmaceutical Press, London, pp 411–915Google Scholar
  3. 3.
    British Pharmacopoeia Commission (2003) British pharmacopeia, vols. I and II. Her Majesty’s Stationery Office, London (through electronic version)Google Scholar
  4. 4.
    Metwally FH, Elzeany EBA, Darwish HW (2005) New methods for determination of cinnarizine in mixture with piracetam by spectrodensitometry, spectrophotometry, and liquid chromatography. J AOAC Int 88:1666–1676PubMedGoogle Scholar
  5. 5.
    Abdine H, Belal F, Zoman N (2002) Simple spectrophotometric determination of cinnarizine in its dosage forms. Farmaco 57:267–271PubMedCrossRefGoogle Scholar
  6. 6.
    El-Gindy A, Emara S, Hadad GM (2004) Determination of certain drugs in binary mixtures formulations by second derivative ratio spectrophotometry and LC. Farmaco 59:703–712PubMedCrossRefGoogle Scholar
  7. 7.
    El-Gindy A, Emara S, Mostafa A (2004) Spectrophotometric and LC determination of two binary mixtures containing antihistamins. Farmaco 59:713–722PubMedCrossRefGoogle Scholar
  8. 8.
    Xu BZ, Zhao FL, Tong SY (1999) Spectrophotometric determination of cinnarizine based on charge-transfer reaction. Guangpuxue-Yu-Guangpu-Fenxi 19:886–888Google Scholar
  9. 9.
    Hassan SSM, Abdel-Aziz RM, Abbas AB (1996) Potentiometric membrane sensors for the selective determination of cinnarizine in pharmaceutical preparations. Anal Chim Acta 321:47–52CrossRefGoogle Scholar
  10. 10.
    Zeng YH, Sun HY (1993) Determination of cinnarizine and its adsorptive voltammetric behaviour. Fenxi-Huaxue 21:1185–1187Google Scholar
  11. 11.
    De Steene JCV, Mortier KA, Lambert WE (2006) Tackling matrix effects during development of a liquid chromatographic–electrospray ionisation tandem mass spectrometric analysis of nine basic pharmaceuticals in aqueous environmental samples. J Chromatogr A 1123:71–81PubMedCrossRefGoogle Scholar
  12. 12.
    Hassan SSM, Elmosallamy MAF, Abbas AB (2002) LC and TLC determination of cinnarizine in pharmaceutical preparations and serum. J Pharm Biomed Anal 28:711–719PubMedCrossRefGoogle Scholar
  13. 13.
    Rosseel MT, Lefebvre RA (1993) Sensitive determination of cinnarizine in human plasma by high-performance liquid chromatography and fluorescence detection. Chromatographia 36:356–358CrossRefGoogle Scholar
  14. 14.
    Sane RT, Sahasrabudhe SP, Nayak VG, Ladage KD, Kothurkar RM, Nayak VG (1989) High-performance liquid-chromatographic determination of cinnarizine from pharmaceutical preparations. Indian Drugs 26:491–493Google Scholar
  15. 15.
    Xie XT, Wu GL, Liu CH (1993) Determination of cinnarizine in tablets by gas chromatography. Sepu 11:315–316Google Scholar
  16. 16.
    Amin AS, Ragab GH (2003) Spectrophotometric methods for the determination of anti-emetic drugs in bulk and in pharmaceutical preparations. Anal Sci 19:747–751PubMedCrossRefGoogle Scholar
  17. 17.
    Rama-Mohan Y, Avadhanulu AB (1998) Extractive spectrophotometric determination of domperidone in its pharmaceutical dosage forms. Indian Drugs 35:754–756Google Scholar
  18. 18.
    Rao GR, Kini GR, Avadhanulu AB, Vatsa DK (1990) Spectrophotometric estimation of acebutolol hydrochloride and domperidone in their dosage forms. East Pharm 33:133–135Google Scholar
  19. 19.
    Al-Khamis KI, Hagga MEM, Al-Khamees HA (1990) Spectrophotometric Determination of Domperidone Using A Method. Anal Lett 23:451–460Google Scholar
  20. 20.
    Baeyens W, De-Moerloose P (1979) Fluorescence properties of domperidone and its determination in pharmaceutical preparations. Anal Chim Acta 110:261–270CrossRefGoogle Scholar
  21. 21.
    Wahdan T, Abd El-Ghany N (2005) Determination of domperidone in tablet dosage form by anodic differential pulse voltammetry. Farmaco 60:830–833PubMedCrossRefGoogle Scholar
  22. 22.
    Zarapkar SS, Salunkhe BB (1990) Determination of domperidone by high-performance thin-layer chromatography in pharmaceutical preparations. Indian Drugs 27:537–540Google Scholar
  23. 23.
    Ali MS, Ghori M, Khatri AR (2006) Stability indicating simultaneous determination of domperidone (DP), methylparaben (MP) and propylparaben by high performance liquid chromatography (HPLC). J Pharm Biomed Anal 41:358–365PubMedCrossRefGoogle Scholar
  24. 24.
    Kobylinska M, Kobylinska K (2000) High-performance liquid chromatographic analysis for the determination of domperidone in human plasma. J Chromatogr B 744:207–212CrossRefGoogle Scholar
  25. 25.
    Zavitsanos AP, MacDonald C, Bassoo E, Gopaul D (1999) Determination of domperidone in human serum and human breast milk by highperformance liquid chromatography-electrospray mass spectrometry. J Chromatogr B 730:9–24CrossRefGoogle Scholar
  26. 26.
    Kanumula GV, Raman B (2000) Simultaneous determination of ranitidine hydrochloride and domperidone in pharmaceutical dosage by reverse-phase high-performance liquid chromatography. Indian Drugs 37:375–378Google Scholar
  27. 27.
    Smit MJ, Sutherland FCW, Hundt HKL, Swart KJ, Hundt AF, Els J (2002) Rapid and sensitive liquid chromatography-tandem mass spectrometry method for the quantitation of domperidone in human plasma. J Chromatogr A 949:65–70PubMedCrossRefGoogle Scholar
  28. 28.
    Oosterveld WJ (1987) The combined effect of cinnarizine and domperidone on vestibular susceptibility. Aviat Space Environ Med 58:218–223PubMedGoogle Scholar
  29. 29.
    Salem MY, El-Bardicy MG, El-Tarras MF, El-Zanfally ES (2002) Simultaneous determination of domperidone maleate and cinnarizine in a binary mixture using derivative ratio spectrophotometry and classical least squares calibration. J Pharm Biomed Anal 30:21–33PubMedCrossRefGoogle Scholar
  30. 30.
    Zarapkar SS, Bhandari NP, Halker UP (2000) Simultaneous estimation of cinnarizine and domperidone maleate in tablets by reversephase HPLC. Indian Drugs 37:295–298Google Scholar
  31. 31.
    Argekar AP, Shah SJ (1999) Simultaneous determination of cinnarizine and domepiridone maleate from tablet dosage form by reverse phase ion pair high performance liquid chromatography. J Pharm Biomed Anal 19:813–817PubMedCrossRefGoogle Scholar
  32. 32.
    Argekar AP, Powar SG (1999) Simultaneous HPTLC determination of cinnarizine and domperidone maleate in formulations. J Planar Chromatogr Mod TLC 12:272–274Google Scholar
  33. 33.
    Chen GZ, Huang XZ, Xu JG, Zheng ZZ, Wang ZB (1990) The methods of fluorescence analysis, 2nd edn. Science, Beijing, p 112Google Scholar
  34. 34.
    Patra D, Mishra AK (2002) Recent developments in multi-component synchronous fluorescence scan analysis. Trends Anal Chem 21:787CrossRefGoogle Scholar
  35. 35.
    Lloyd JBF (1971) Nat Phys Sci 231:64 http://www.chemistrymag.org/cji/2003/058065pe.htm. 2003 Vol.5 No.8 P.65.
  36. 36.
    Murillo Pulgarín JA, Alañón Molina A, Fernández López P (1998) Simultaneous determination of atenolol, propranolol, dipyridamole and amiloride by means of nonlinear variable-angle synchronous fluorescence spectrometry. Anal Chim Acta 370:9–18CrossRefGoogle Scholar
  37. 37.
    Aodeng GW, Zhang Y, Fan HY (2006) Determination of metacycline in mixture sample by synchronous-derivative fluorimetry. Guang Pu Xue Yu Guang Pu Fen Xi 26:1530–1532PubMedGoogle Scholar
  38. 38.
    Karim MM, Jeon CW, Lee HS, Alam SM, Lee SH, Choi JH, Jin SO, Das AK (2006) Simultaneous determination of acetylsalicylic acid and caffeine in pharmaceutical formulation by first derivative synchronous fluorimetric method. J Fluoresc 16:713–721PubMedCrossRefGoogle Scholar
  39. 39.
    Wei YF, Li XH, Ma DM (2005) Simultaneous determination of aspirin and salicyclic acid by synchronous fluorescence spectrometry. Guang Pu Xue Yu Guang Pu Fen Xi 25:588–590PubMedGoogle Scholar
  40. 40.
    Murillo Pulgarín JA, Alañón Molina A, Fernández López P, Sánchez-Ferrer Robles I (2007) Direct determination of closely overlapping drug mixtures of diflunisal and salicylic acid in serum by means of derivative matrix isopotential synchronous fluorescence spectrometry. Anal Chim Acta 583:55–62PubMedCrossRefGoogle Scholar
  41. 41.
    Xiao Y, Wang HY, Han J (2005) Simultaneous determination of carvedilol and ampicillin sodium by synchronous fluorimetry. Acta A Mol Biomol Spectrosc 61:567–573CrossRefGoogle Scholar
  42. 42.
    Fernandez-González R, Garcýa-Falcón MS, Simal-Gándara J (2002) Quantitative analysis for oxytetracycline in medicated premixes and feeds by second-derivative synchronous spectrofluorimetry. Anal Chim Acta 455:143–148CrossRefGoogle Scholar
  43. 43.
    Toma’s PR, Carmen ML, Virginia T, Jose C (1998) Analysis of binary mixtures of flufenamic, meclofenamic and mefenamic acids by derivative synchronous fluorescence spectrometry Talanta 47:537–545CrossRefGoogle Scholar
  44. 44.
    Sun Y, Nakashima MN, Takahashi M, Kuroda N, Nakashima K (2002) Determination of bisphenol A in rat brain by microdialysis and column switching high-performance liquid chromatography with fluorescence detection Biomed Chromatogr 16:319–326PubMedCrossRefGoogle Scholar
  45. 45.
    US Department of Health and Human Services, Food and Drug Administration (2001) Guidance for industry bioanalytical method validation. Center for Drug Evaluation and Research, Rockville. http://www.fda.gov/eder/guidance/4252fnl.pdf (accessed September 1, 2004)
  46. 46.
    Miller JC, Miller JN (1993) Statistics for analytical chemistry, 3rd edn. Wiley, New York, p 115Google Scholar
  47. 47.
    Moffat AC, Osselton MD, Widdop B, Galichet LY (2004) Clark’s analysis of drugs and poisons in pharmaceuticals, body fluids and postmortem material, 3rd edn., vol II. The Pharmaceutical Press, London, pp 925, 1695Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • M. I. Walash
    • 1
  • F. Belal
    • 1
  • N. El-Enany
    • 1
  • A. A. Abdelal
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of PharmacyUniversity of MansouraMansouraEgypt

Personalised recommendations