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Journal of Fluorescence

, Volume 17, Issue 6, pp 721–726 | Cite as

Study on the Interaction between Florasulam and Bovine Serum Albumin

  • Dehuan Ran
  • Xia Wu
  • Jinhua Zheng
  • Jinghe Yang
  • Haiping Zhou
  • Meifeng Zhang
  • Yongjun Tang
Original Paper

Abstract

In this paper, the interaction between florasulam (FU, 2′,6′,8-trifluoro-5-methoxy [Kragh-Hansen U, Molecular aspects of ligand binding to serum albumin. Pharmacol Rev 33(1):17–53 1981; Carter DC and Ho JX, Structure of serum albumin. Adv Protein Chem 45:153–203 1994; He XM, and Carter DC, Atomic structure and chemistry of human serum albumin. Nature 358(6383):209–215 1992] triazolo [1,5-c]pyrimidine-2-sulfonanilide) and bovine serum albumin (BSA) was investigated by fluorescence, ultraviolet absorption (UV) and Far-UV circular dichroism (CD) spectrometries. A strong fluorescence quenching was observed and the quenching mechanism was considered as static quenching. The binding constant of FU with BSA at 299 and 309 K were obtained as 1.5 × 104 and 7.1 × 103 l mol−1, respectively. There was one binding site between FU and BSA. The thermodynamic parameters enthalpy change (ΔH) and entropy change (ΔS) were calculated as −57.89 kJ mol−1 and −113.6 J mol−1 K−1, respectively, which indicated that the acting force between FU and BSA was mainly hydrogen bond and Van der Waals force. According to the Förster non-radiation energy transfer theory, the average binding distance between donor (BSA) and acceptor (FU) was obtained (r = 1.59 nm). The investigations of the UV/Vis and CD spectra of the system showed that the conformation of BSA was changed in presence of FU.

Keywords

Florasulam Bovine serum albumin Fluorescence quenching Förster non-radiative energy transfer 

Notes

Acknowledgements

This work is supported by the Natural Science Foundations of China (20575035) and Shandong Province (Y2003B02).

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Key Laboratory of Colloid and Interface Chemistry (Shandong University)Ministry of Education, School of Chemistry and Chemical Engineering, Shandong UniversityJinanPeople’s Republic of China
  2. 2.Shandong Pesticide Research InstituteJinanPeople’s Republic of China

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