Spectrofluorimetric Determination of Dopamine Using Chlorosulfonylthenoyltrifluoroacetone–Europium Probe
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A new spectrofluorimetric method was developed for the determination of trace amounts of dopamine (DA). Using chlorosulfonylthenoyltrifluoroacetone (CTTA)–europium ion (Eu3+) as a fluorescent probe, in a buffer solution at pH = 10.0, DA can remarkably enhance the fluorescence intensity of the CTTA-Eu3+ complex at λ = 612 nm; the enhanced fluorescence intensity of Eu3+ is proportional to the concentration of DA. Optimum conditions for the determination of DA were also investigated. The linear range and detection limit for the determination of DA were 5.0 × 10−8∼1.6 × 10−5 mol/l and 3.2 × 10−8 mol/l. This method is simple, practical and relatively free of interference from coexisting substances, and can be applied to assess DA in injection and human serum samples with good precision and accuracy.
KeywordsDopamine Chlorosulfonylthenoyltrifluoroacetone Europium Spectrofluorimetry
- 9.Yoshitake T, Kehr J, Yoshitake S, Fujino K, Nohta H, Yamaguchi M (2004) Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine. J Chromatogr B 807:177–183CrossRefGoogle Scholar
- 23.China Pharmacopoeia Committee (2005) Pharmacopoeia of the People’s Republic of China, the second part. Chemical Industry Press, BeijingGoogle Scholar