Fluorescence Lifetime Correlation Spectroscopy
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This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using common terms and minimum mathematics. The usefulness of the method is demonstrated on simple FCS experiments. The method makes possible to separate the autocorrelation function of individual components of a mixture of fluorophores, as well as purging the result from parasitic contributions like scattered light or detector afterpulsing.
KeywordsFCS TCSPC Lifetime Correlation Multichannel detection Scattering Afterpulsing
A.B. and M.H. acknowledge financial support by the Ministry of Education and Sport of the Czech Republic via grant LC06063.
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