Journal of Fluorescence

, Volume 16, Issue 4, pp 581–587

The Calibration Kit Spectral Fluorescence Standards—A Simple and Certified Tool for the Standardization of the Spectral Characteristics of Fluorescence Instruments

  • D. Pfeifer
  • K. Hoffmann
  • A. Hoffmann
  • C. Monte
  • U. Resch-Genger
Original Paper

Abstract

With the Calibration Kit Spectral Fluorescence Standards BAM-F001–BAM-F005, we developed a simple tool for the characterization of the relative spectral responsivity and the long-term stability of the emission channel of fluorescence instruments under routine measurement conditions thereby providing the basis for an improved comparability of fluorescence measurements and eventually standardization. This first set of traceable fluorescence standards, which links fluorescence measurements to the spectral radiance scale in the spectral range of 300–770 nm and has been optimized for spectrofluorometers, can be employed for different measurement geometries and can be adapted to different fluorescence techniques with proper consideration of the underlying measurement principles.

Keywords

Fluorescence Standard Spectral correction Emission Calibration tool 

References

  1. 1.
    Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer Academic/Plenum, New YorkGoogle Scholar
  2. 2.
    Lakowicz JR (ed) (1992–2004) Topics in fluorescence spectroscopy, series vol 1–8. Plenum, New YorkGoogle Scholar
  3. 3.
    Wolfbeis OS (series ed) (2001–2004) Springer series on fluorescence, methods and applications, vol 1–3. Springer, BerlinGoogle Scholar
  4. 4.
    Schulman SG (ed) (1985–1993) Molecular luminescence spectroscopy parts 1–3. Wiley Interscience, New YorkGoogle Scholar
  5. 5.
    Mason WT (1999) Fluorescent and luminescent probes for biological activity, 2nd edn. Academic, San DiegoGoogle Scholar
  6. 6.
    Resch-Genger U, Hoffmann K, Nietfeld W, Engel A, Neukammer J, Nitschke R, Ebert B, Macdonald R (2005) How to improve quality assurance in fluorometry: fluorescence-inherent sources of error and suited fluorescence standards. J Fluoresc 15(3):337–362 and references thereinPubMedCrossRefGoogle Scholar
  7. 7.
    Monte C, Resch-Genger U, Pfeifer D, Taubert RD, Hollandt J (2005) Linking fluorescences measurements to radiometric units, Metrologia (in press)Google Scholar
  8. 8.
    Resch-Genger U, Pfeifer D, Monte C, Pilz W, Hoffmann A, Spieles M, Rurack K, Hollandt J, Taubert D, Schönenberger B, Nording P (2005) Traceability in fluorometry: part II. Spectral fluorescence standards. J Fluoresc 15(3):315–336PubMedCrossRefGoogle Scholar
  9. 9.
    Certificate of the Federal Institute for Materials Research and Testing (BAM) (2006) Certified Reference Materials BAM-F001, BAM-F002, BAM-F003, BAM-F004, and BAM-F005, Calibration Kit Spectral Fluorescence Standards, for the determination of the relative spectral responsivity of fluorescence instruments, January 10, 2006Google Scholar
  10. 10.
    Hollandt J, Taubert DR, Seidel J, Resch-Genger U, Gugg-Helminger A, Pfeifer D Monte C (2005) Traceability in fluorometry: part I physical standards. J Fluoresc 15(3):301–313PubMedCrossRefGoogle Scholar
  11. 11.
    Eastman JW (1966) Standardization of fluorescence spectra and the calibration of spectrofluorimeters. Appl Opt 5(7):1125–1132CrossRefGoogle Scholar
  12. 12.
    Miller JN (1981) Standards in fluorescence spectrometry. Ultraviolett Spectrometry Group, LondonGoogle Scholar
  13. 13.
    Velapoldi RA, Tonnesen HH (2004) Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region. J Fluoresc 14(4):465–472PubMedCrossRefGoogle Scholar
  14. 14.
    Hofstraat JW, Latuhihin MJ (1994) Correction of fluorescence-spectra. Appl Spectrom 48(4):436–447CrossRefGoogle Scholar
  15. 15.
    Hoffmann K, Monte C, Pfeifer D, Resch-Genger U (2005) Standards in fluorescence spectroscopy: simple tools for the characterization of fluorescence instruments. GIT Lab J 5(3):2–4Google Scholar
  16. 16.
    Velapoldi RA, Mielenz KD (1980) A fluorescence standard reference material: quinine sulfate dihydrate. NBS Special Publication, PB 80132046, Springfield, VA, pp 260-264Google Scholar
  17. 17.
    Gardecki JA, Maroncelli M (1998) Set of secondary emission standards for calibration of the spectral responsivity in emission spectroscopy. Appl Spectrom 52(9):1179–1189CrossRefGoogle Scholar
  18. 18.
    Hoffmann K, Resch-Genger U, Nitschke R (2005) Simple Tool for the Standardization of Confocal Spectral Imaging Systems. GIT Imaging Microsc (3):18–19Google Scholar
  19. 19.
    Bremser W, de Rose P, Duewer DS, Ebert B, Hoffmann A, Hollandt J, Macdonald R, Monte C, Pfeifer D, Resch-Genger U, Taubert DR, Zwinkels J (manuscript in preparation)Google Scholar
  20. 20.
    Pfeifer D, Hoffmann A, Monte C, Spieles M, Bremser W, Resch-Genger U (manuscript in preparationGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • D. Pfeifer
    • 1
  • K. Hoffmann
    • 1
  • A. Hoffmann
    • 1
  • C. Monte
    • 1
  • U. Resch-Genger
    • 1
  1. 1.Federal Institute for Materials Research and TestingWorking Group Optical Spectroscopy, Division I.5BerlinGermany

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