Annealed Silver-Island Films for Applications in Metal-Enhanced Fluorescence: Interpretation in Terms of Radiating Plasmons
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The effects of thermally annealed silver island films have been studied with regard to their potential applicability in applications of metal-enhanced fluorescence, an emerging tool in nano-biotechnology. Silver island films were thermally annealed between 75 and 250°C for several hours. As a function of both time and annealing temperature, the surface plasmon band at ≈420 nm both diminished and was blue shifted. These changes in plasmon resonance have been characterized using both absorption measurements, as well as topographically using Atomic Force Microscopy. Subsequently, the net changes in plasmon absorption are interpreted as the silver island films becoming spherical and growing in height, as well as an increased spacing between the particles. Interestingly, when the annealed surfaces are coated with a fluorescein-labeled protein, significant enhancements in fluorescence are osbserved, scaling with annealing temperature and time. These observations strongly support our recent hypothesis that the extent of metal-enhanced fluorescence is due to the ability of surface plasmons to radiate coupled fluorophore fluorescence. Given that the extinction spectrum of the silvered films is comprised of both an absorption and scattering component, and that these components are proportional to the diameter cubed and to the sixth power, respectively, then larger structures are expected to have a greater scattering contribution to their extinction spectrum and, therefore, more efficiently radiate coupled fluorophore emission. Subsequently, we have been able to correlate our increases in fluorescence emission with an increased particle size, providing strong experiment evidence for our recently reported metal-enhanced fluorescence, facilitated by radiating plasmons hypothesis.
KeywordsMetal-enhanced fluorescence radiative decay rate increased excitation rate radiative decay engineering surface-enhanced fluorescence silver island films fluorescence spectroscopy
Atomic Force Microscopy
Human Serum Albumin
Radiative Decay Engineering
Silver Island Films
In the last five or so years our laboratories have both introduced and demonstrated many applications of metal-enhanced fluorescence [1, 2, 3]. These have included the increased detectability and photostability of fluorophores [4, 5, 6], improved DNA detection , the release of self-quenched fluorescence of overlabeled proteins , enhanced wavelength-ratiometric sensing , and the application of metallic surfaces to amplified assay detection [1,10], to name but just a very few. In addition, we have developed many surfaces for metal-enhanced fluorescence [3,11, 12, 13, 14, 15], such as those comprised of silver islands [5,11], silver colloids , silver nanotriangles , silver nanorods , and even fractal-like silvered surfaces . Several modes of silver deposition have also been developed, such as silver deposition by light  and electrochemically  on glass , plastics , and even electrodes .
Similarly, it has recently been hypothesized that metal-enhanced fluorescence is also plasmon coupled  and while not highly directional, as is the case with SPCE, is likely to be dependent on the plasmon scattering properties of the particulate nanostructures .
In this paper we have studied the metal-enhanced fluorescence phenomenon from thermally annealed silver island films, SiFs. By annealing the surfaces we have been able to produce thermally stable SiFs, which are not prone to further temperature effects. This suggests the re-use of these surfaces after autoclaving, i.e., a reusable generic surface for metal-enhanced fluorescence, or indeed, the use of these substrates for applications employing elevated temperatures, such as in MEF hybridization assays . We have also chosen to anneal our freshly prepared silver island films in a conventional laboratory oven in the presence of oxygen. While this process is well-known to rapidly form oxide layers, particularly at high annealing temperatures, we have chosen this procedure so as keep our silver island film preparations consistent with all our other reports of metal-enhanced fluorescence [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19]. In these reports, the silver nanostructures were all fabricated under similar atmospheric conditions, where silver oxide layers are likely to have also readily formed [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19].
In addition, the annealed surfaces produce larger fluorescence enhancements, an ≈50% increase as compared to films, which have not been thermally annealed. We rationale these increases in fluorescence of fluorophore-coated annealed films, as compared to the unannelaed films, in terms of the larger particles which are formed upon annealing, and their subsequent greater scattering cross sections and therefore greater ability to enhance fluorescence, as compared to smaller, unannealed SiFs.
Materials and Methods
Silver nitrate (99.9%), sodium hydroxide (99.996%), ammonium hydroxide (30%), trisodium citrate, D-glucose, and premium quality APS-coated glass slides (75 mm × 25 mm) were obtained from Sigma-Aldrich. All chemicals were used as received.
Formation of Silver Island Films (SiFs) on APS-Coated Glass Substrates
Synthesis of Silver Colloids and Preparation of Silver Colloid Films
Two milliliters of 1.16 mM trisodium citrate solution was added dropwise to a heated (95°C) aqueous solution of 0.65 mM of silver nitrate while stirring. The mixture was kept heated for 10 min, and then it was cooled to room temperature.
Silver colloid films were prepared by immersing the APS-coated glass slides in silver colloid solution overnight. The silver colloid deposited slides were rinsed with deionized water several times prior to the annealing and fluorescence experiments.
Annealing of Silver Island Films and Silver Colloid Films
Annealing of silver island films and silver colloid films were performed by placing the silver-coated slides in a Thelco Laboratory Oven at various temperatures (75, 121, 190, and 250°C) for up to 10 hr.
Metal-Enhanced Fluorescence from Annealed Silver Island Films and Silver Colloids
In previous reports of metal-enhanced fluorescence (MEF), our laboratories have coated silvered surfaces with fluorophore-labeled protein [1, 2, 3]. This experimental format has been adopted for two main reasons, the first, being that the protein coverage with Human Serum Albumin (HSA) is known to bind to silvered surfaces and indeed forms a monolayer [1, 2, 3,11] and secondly, the dimensions of the protein being such that the protein allows for a mean ≈4 nm separation of the silver and the fluorophore, MEF being a through space phenomenon, as demonstrated by the late T. Cotton and indeed our laboratories [1, 2, 3]. In contrast, Surface-Enhanced Raman Scattering (SERS) is known to be a consequence of mostly contact between the species of interest and the silvered surface [1, 2, 3, 4].
Binding the FITC-HSA to the silver island films and silver colloid films was accomplished by soaking in a 10 µM FITC-HSA solution for 2 hr, followed by rinsing with water to remove the unbound material. Both the unsilvered and silvered films were coated with labeled HSA, which is known to passively absorb to noble metal surfaces and form a ≈4 nm thick protein monolayer [1, 2, 3], allowing us to study the fluorescence spectral properties of noncovalent FITC-HSA complexes in the absence and presence of SiFs. By equally coating a silver film with FITC-HSA we were also able to determine the enhancement factor (benefit) obtained from using the silver, i.e., Intensity on Silver/Intensity on Unsilvered glass, given that both surfaces are known to have an ≈ equal monolayer coverage [1, 2, 3,11].
Characterization of Annealed Silver Island Films and Silver Colloid Films
All absorption measurements were performed using a HP 8453 UV-Vis spectrophotometer. Fluorescence measurements on silver island films and silver colloid films were performed using a Varian Cary fluorescence spectrophotometer by placing the films on a stationary stage (positioned in front of the excitation source and the detector) equipped with mirrors to divert the excitation/emission to and from the sample.
AFM images were collected with an Atomic Force Microscope (TMX 2100 Explorer SPM, Veeco) equipped with an AFM dry scanner (the scanning area was 100 µm × 100 µm). Surfaces were imaged in air, in a tapping mode of operation, using SFM noncontact mode cantilevers (Veeco). The AFM scanner was calibrated using a standard calibration grid as well as by using gold nanoparticles, 100 nm in diameter from Ted Pella. Images were analyzed using SPMLab software.
Time-resolved intensity decays were measured using reverse start-stop time-correlated single-photon counting (TCSPC)  with a Becker and Hickl gmbh 630 SPC PC card and an unamplified MCP-PMT. Vertically polarized excitation at ≈440 nm was obtained using a pulsed laser diode, 1 MHz repetition rate.
Results and Discussion
These changes in SiF morphology on surfaces have also been observed by others, [26, 27, 28, 29, 30, 31, 32, 33] while developing platforms for Surface-Enhanced Raman Scattering [31, 32, 33]. Similar to previous interpretations, our changes in plasmon extinction, coupled with an insight into the size and shape changes revealed by AFM, can be explained by net surface plasmon changes. While an increased particle size would be expected to result in a red shifted plasmon absorption maximum [34, 35, 36], the dominant influence here is the decreased interaction of neighboring SiFs, as a result of an increased particle separation, noting that one would expect mass to be conserved on the surface during annealing. The interparticle dipole-dipole interactions are known to determine the width of the extinction bands of SiFs . Hence, as the SiFs become spherical, larger, and more spaced, the net change in silver plasmon absorption is a blue shifted and decreased absorption maximum. Interestingly for films annealed at 250°C, then a slightly different behavior is observed, whereby the silver plasmon absorption eventually red shifts after initially shifting blue. This is thought to be due to the particles being sufficiently spaced that the dipole-dipole interactions become very weak, the plasmon absorption now dominated by the size and shape of the isolated particles.
Finally, it is interesting to note that the annealed films yield both increased emission from locally positioned fluorescein, as well as a reduced fluorescein lifetime. As briefly mentioned in the Introduction, this combination of an increased quantum yield, coupled with a decreased lifetime is unusual in fluorescence spectroscopy, and is consistent with a modification of the intrinsic radiative decay rate of the fluorophore, see Eqs. (1–4). This forms the basis of our earlier interpretations of metal-enhanced fluorescence [1, 2, 3, 4, 5,20].
Metal-Enhanced Fluorescence: Interpretation in Terms of Radiating Plasmons
Until recently the emission of fluorophores in close proximity to metallic nanostructures was thought to originate solely from the fluorophore, the excited plasmons interacting with the fluorophore and changing its free-space spectral characteristics [1, 2, 3, 4, 5,20]. However, recently our interpretation of metal-enhanced fluorescence has changed somewhat  as shown in Fig. 1, to one whereby excited fluorophores can nonradiatively transfer energy to surface plasmons which in turn, radiate the fluorophores’ photophysical characteristics, in essence the system radiates . While there is very little experimental evidence to date that themetal-enhanced fluorescence phenomenon is due to fluorophore-coupled radiating plasmons, the data presented here for thermally annealed silver island films, certainly goes some ways to support this interpretation, as is described below.
It is known that surface plasmons can be created by illumination of thin continuous metal films under very unique optical conditions, such as through a prism, or a medium of high dielectric constant and with p-polarized light . However, surface plasmons can also be created by direct illumination of metallic solution based colloids or nanostructures, or even by nanostructures bound to surfaces . Illumination of nanostructures or colloids typically results in the visualization of strong colors, which is due to a combination of both absorption and scattering [35,36]. The term “absorption” is generically used for these nanostructures and colloids, but the correct term is “extinction,” as there are both absorption and scattering components to the observed colors. Based on the recent radiating plasmon model postulated by our laboratories , small colloids are expected to quench fluorescence, because the absorption component of the extinction is dominant over scattering, while larger colloids or nanostructures are expected to result in enhanced fluorescence, as the scattering component is now dominant over the absorption component of the extinction spectra. Intuitively, by considering Eqs. (5) and (6) we expect the absorption term CA to cause quenching, and the scattering term CS to cause fluorescence enhancement due to fluorophore coupling (described in the next section in more detail). Examination of Eq. (5) shows that CA increases as the radius of the nanoparticle cubed, whereas CS increases as the radius to the sixth power. For this reason, larger nanostructures are expected to show greater fluorescence enhancements than smaller nanoparticles. While this understanding underpins Mie theory [35,36,38, 39, 40] for small spherical particles whose radius is less than 0.05 λ, the general conditions and equations are still valid for much larger nanostructures, but considerably more complex [35,36,38, 39, 40].
With regard to the fluorescence enhancements observed here for annealed silver island films, it is thought that the fluorescence enhancement increase, Fig. 7 bottom, is due to the increased size of the nanostructures after annealing, the CS component of the extinction becoming more dominant than the absorption, CA, component. Similarly, the annealed colloidal film data also bears out this interpretation, as the size of the surface-bound colloids remained approximately the same after annealing, the Fluorescein-HSA enhancement approximately constant as seen in Fig. 7 bottom. In addition, while the electric field effect or sometimes called the “lightening rod effect” [4,20] is known to modify fluorophore absorption cross sections, thereby increasing the excitation rate of fluorophores, this effect is dependent on the close locality of the fluorophore to the nanostructures, and would not be expected to increase with an increase in interparticle spacing, as is observed here. Moreover, a modification in the excitation rate of fluorophores would not alter a fluorophores’ fluorescence lifetime [4,20]. To the best of our knowledge this is the first experimental observation that supports the hypothesis of fluorophore-plasmon coupled emission from noncontinuous particulate films.
An Increased Quantum Yield, Decreased Lifetime, and Plasmon-Coupled Emission
While we have rationaled the observations of enhanced fluorescence intensities as a function of SiF annealing in terms of the increased CS component of the extinction spectra, it is informative to comment on the quantum yield and lifetime changes observed, given that current thinking has slightly shifted from a radiative rate modification, as originally depicted by Eqs. (1–4) [1, 2, 3].
The lifetime of surface plasmons are known to be very short, on the order of tens of femtoseconds [41,42]. This suggests that in our radiating plasmon interpretation, the energy transfer is essentially one way, from fluorophore to metal . The increased quantum yield of fluorophores in close proximity to metallic nanostructures can be understood as the result of rapid energy transfer to the plasmons, which then radiate to the far-field . When discussing excited fluorophores near to metals, we assume that the near field is present while the fluorophore is in an excited state, i.e., a field around an oscillating dipole at distances closer than the wavelength. For far field radiation, we refer to a wave propagating away from its source, whether fluorophore or metal. For a fluorophore, the far-field wave exists after it releases a photon and returns to the ground state.
The concept of donor-acceptor emission was first described by Forster for donor-acceptor pairs . However, our laboratories have recently shown experimentally for fluorophores that rapid energy transfer from a donor to an acceptor resulted in an overall increase in the quantum yield of the system, when the quantum yield of the acceptor is greater than the donor [43,44]. This effect occurs because the rate of Forster energy transfer is proportional to the radiative decay rate of the donor and is independent of the nonradiative decay rates. If the transfer rate is high, which is thought to be the case for fluorophore-metal combinations, then the energy is transferred before the donor can decay by the nonradiative pathways, which are inherently the same in the absence and presence of the acceptor.
As the rate of transfer becomes larger than the inverse lifetime, then the transfer efficiency approaches unity.
This means that for fluorophore-metal combinations, the effective quantum yield of the fluorophore approaches unity and the overall quantum yield of the system becomes the quantum yield of scattering. Remarkably, this occurs irrespective of whether the fluorophore has a low or high quantum yield. This forms the basis of our recent radiating plasmon model .
In terms of the annealed SiF data presented in this paper, we see an overall increase in the fluorescence intensity as the size of the nanostructures increases, because the quantum yield of scattering is higher for larger structures than for smaller structures, i.e., competition between absorption (quenching) and scattering, the CA = radius3 vs. CS =radius6, c.f. Eqs. (5) and (6) [35,36]. The lifetime of fluorescein in the presence of metal also drops, similar to any donor in the presence of an efficient acceptor as depicted by Forster’s theory .
In this paper we have shown that silver island films can undergo both size and shape changes as a function of thermal annealing between 75 and 250°C. In contrast, silver colloid films, were relatively unperturbed by thermal annealing. When coated with a fluorophore-labeled protein, then metal-enhanced fluorescein fluorescence can be observed from the surfaces, up to a 10-fold increase as compared to surfaces having no silver. These findings suggest the use of annealed silver island films as reusable autoclavable substrates for clinical assays, or for elevated temperature-based sensing, such as for use in metal-enhanced fluorescence hybridization assays.
We have also rationaled the increases in fluorescence observed, not in terms of a fluorophore radiative decay rate modification as was previously postulated by our laboratories, but one which involves efficient energy transfer to surface plasmons, which then radiate, depending on the quantum efficiency of the scattering component of the particles’ extinction spectrum, which is itself dependent on nanoparticle size. While the data presented here is not definitive proof of our new interpretation of metal-enhanced fluorescence, the size dependence of the silver nanostructures on fluorescence enhancement, certainly supports our interpretation and the new radiating plasmon model.
Finally, metal-enhanced fluorescence is a new and emerging tool in biotechnology. At present it is difficult to predict whether a particular sized metal structure will quench or even enhance fluorescence. Subsequently, more detailed studies of the sizes and shapes of nanostructures, and the locality of fluorophores are needed with regard to quantitative MEF. In this regard, the radiating plasmon model provides the first framework for this interpretation. Further studies by our laboratories are currently underway.
This work was supported by the NIHGM070929 and RR008119. Partial salary support to CDG and JRL from UMBI is also acknowledged.
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