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Journal of Chemical Ecology

, Volume 45, Issue 8, pp 673–683 | Cite as

Seasonal Variations in Femoral Gland Secretions Reveals some Unexpected Correlations Between Protein and Lipid Components in a Lacertid Lizard

  • Marco MangiacottiEmail author
  • Stefano Pezzi
  • Marco Fumagalli
  • Alan Jioele Coladonato
  • Patrizia d’Ettorre
  • Chloé Leroy
  • Xavier Bonnet
  • Marco A. L. Zuffi
  • Stefano Scali
  • Roberto Sacchi
Article
  • 29 Downloads

Abstract

Animals modulate intraspecific signal shape and intensity, notably during reproductive periods. Signal variability typically follows a seasonal scheme, traceable through the expression of visual, acoustic, chemical and behavioral patterns. The chemical channel is particularly important in lizards, as demonstrated by well-developed epidermal glands in the cloacal region that secrete lipids and proteins recognized by conspecifics. In males, the seasonal pattern of gland activity is underpinned by variation of circulating androgens. Changes in the composition of lipid secretions convey information about the signaler’s quality (e.g., size, immunity). Presumably, individual identity is associated with a protein signature present in the femoral secretions, but this has been poorly investigated. For the first time, we assessed the seasonal variability of the protein signal in relation to plasma testosterone level (T), glandular activity and the concentration of provitamin D3 in the lipid fraction. We sampled 174 male common wall lizards (Podarcis muralis) over the entire activity season. An elevation of T was observed one to two months before the secretion peak of lipids during the mating season; such expected delay between hormonal fluctuation and maximal physiological response fits well with the assumption that provitamin D3 indicates individual quality. One-dimensional electrophoretic analysis of proteins showed that gel bands were preserved over the season with an invariant region; a result in agreement with the hypothesis that proteins are stable identity signals. However, the relative intensity of bands varied markedly, synchronously with that of lipid secretion pattern. These variations of protein secretion suggest additional roles of proteins, an issue that requires further studies.

Keywords

Chemical communication Season Testosterone Quality Identity Femoral glands Cosinor models Lizards Podarcis muralis 

Notes

Acknowledgments

The study was performed in accordance with the European and Italian laws on animal use in scientific research, and all the protocols have been authorized by Italian Environmental Ministry (Aut. Prot. PNM-2015-0010423, PNM-2016-0002154). This research was funded by the FRG_2016 (Italian Ministry of Education, University and Research - MIUR) to Roberto Sacchi. We would like to thank Matteo Panaccio for his help during fieldwork, Lorenzo Balestrazzi for his help in lab analysis, the Associate Editor and two anonymous reviewers for their constructive comments on the early version of the manuscript. The authors declared no competing interests.

Supplementary material

10886_2019_1092_MOESM1_ESM.pdf (110 kb)
ESM 1 (PDF 110 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Marco Mangiacotti
    • 1
    • 2
    Email author
  • Stefano Pezzi
    • 1
  • Marco Fumagalli
    • 3
  • Alan Jioele Coladonato
    • 1
  • Patrizia d’Ettorre
    • 4
  • Chloé Leroy
    • 4
  • Xavier Bonnet
    • 5
  • Marco A. L. Zuffi
    • 6
  • Stefano Scali
    • 2
  • Roberto Sacchi
    • 1
  1. 1.Department of Earth and Environmental SciencesUniversity of PaviaPaviaItaly
  2. 2.Museo di Storia Naturale di MilanoMilanItaly
  3. 3.Department of Biology and Biotechnologies “L. Spallanzani”, Unit of BiochemistryUniversity of PaviaPaviaItaly
  4. 4.LEEC Laboratoire d’Ethologie Expérimentale et ComparéeVilletaneuseFrance
  5. 5.Centre d’Etudes Biologiques de ChizéCNRS UMR 7372 - Université de La RochelleVilliers-en-BoisFrance
  6. 6.Museo di Storia Naturale dell’Università di PisaCalciItaly

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