Journal of Chemical Ecology

, Volume 41, Issue 9, pp 822–836 | Cite as

Phenolic Compounds and Their Fates In Tropical Lepidopteran Larvae: Modifications In Alkaline Conditions

  • Matti Vihakas
  • Isrrael Gómez
  • Maarit Karonen
  • Petri Tähtinen
  • Ilari Sääksjärvi
  • Juha-Pekka Salminen
Article

Abstract

Lepidopteran larvae encounter a variety of phenolic compounds while consuming their host plants. Some phenolics may oxidize under alkaline conditions prevailing in the larval guts, and the oxidation products may cause oxidative stress to the larvae. In this study, we aimed to find new ways to predict how phenolic compounds may be modified in the guts of herbivorous larvae. To do so, we studied the ease of oxidation of phenolic compounds from 12 tropical tree species. The leaf extracts were incubated in vitro in alkaline conditions, and the loss of total phenolics during incubation was used to estimate the oxidizability of extracts. The phenolic profiles of the leaf extracts before and after incubation were compared, revealing that some phenolic compounds were depleted during incubation. The leaves of the 12 tree species were each fed to 12 species of lepidopteran larvae that naturally feed on these trees. The phenolic profiles of larval frass were compared to those of in vitro incubated leaf extracts. These comparisons showed that the phenolic profiles of alkali-treated samples and frass samples were similar in many cases. This suggested that certain phenolics, such as ellagitannins, proanthocyanidins, and galloylquinic acid derivatives were modified by the alkaline pH of the larval gut. In other cases, the chromatographic profiles of frass and in vitro incubated leaf extracts were not similar, and new modifications of phenolics were detected in the frass. We conclude that the actual fates of phenolics in vivo are often more complicated than can be predicted by a simple in vitro method.

Keywords

Amazonia Faeces MRM Neotropical Oxidation Rain forest SRM UHPLC–DAD–MS 

Supplementary material

10886_2015_620_MOESM1_ESM.docx (650 kb)
ESM 1(DOCX 649 kb)
10886_2015_620_MOESM2_ESM.doc (340 kb)
ESM 2(DOC 339 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Matti Vihakas
    • 1
  • Isrrael Gómez
    • 2
  • Maarit Karonen
    • 1
  • Petri Tähtinen
    • 1
  • Ilari Sääksjärvi
    • 2
  • Juha-Pekka Salminen
    • 1
  1. 1.Laboratory of Organic Chemistry and Chemical Biology, Department of ChemistryUniversity of TurkuTurkuFinland
  2. 2.Zoological Museum, Department of BiologyUniversity of TurkuTurkuFinland

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